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目的探讨CD40基因启动子区-1C/T单核苷酸多态性位点(SNP)与冠状动脉(冠脉)不稳定粥样斑块的相关性。方法 474例冠心病(CHD)患者冠脉造影(CAG)粥样斑块形态学分型为Ⅰ型(131例)、Ⅱ型(179例)和Ⅲ型(164例),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测CD40基因多态性及测序鉴定;并与225例CAG正常者对照。结果 C等位基因频率CHD组明显高于对照组(52.6%vs.42.8%)(P<0.05),而T等位基因频率CHD组明显低于对照组(20.3%vs.32.0%)(P<0.05);CHDⅠ、Ⅱ、Ⅲ亚组C等位基因频率分别为43.1%、56.4%、56.1%,T等位基因频率分别为56.9%、43.6%、43.9%,Ⅱ型、Ⅲ型亚组C等位基因频率高于Ⅰ型亚组,而T等位基因频率低于Ⅰ型亚组(P<0.05)。结论 CD40-1C/TSNP位点与冠脉斑块的不稳定性相关,C等位基因增加了斑块的易损风险。
Objective To investigate the association between CD40 gene promoter-1 C / T single nucleotide polymorphism (SNP) and coronary atherosclerotic plaque. Methods A total of 474 patients with coronary heart disease (CHD) underwent coronary angiography (CAG) atherosclerotic plaque morphology type Ⅰ (131 cases), type Ⅱ (179 cases) and type Ⅲ (164 cases). Polymerase chain reaction - The polymorphism of CD40 gene was detected by PCR-RFLP and sequenced. The results were compared with 225 normal CAG patients. Results The frequency of C allele in CHD group was significantly higher than that in control group (52.6% vs.42.8%) (P <0.05), but the frequency of T allele in CHD group was significantly lower than that in control group (20.3% vs.32.0%) <0.05). The frequencies of C allele in subgroups of CHD Ⅰ, Ⅱ and Ⅲ were 43.1%, 56.4% and 56.1%, respectively. The frequency of T allele was 56.9%, 43.6% and 43.9% The frequency of C allele was higher than that of type Ⅰ subgroup, but the frequency of T allele was lower than that of type Ⅰ subgroup (P <0.05). Conclusion The CD40-1C / TSNP locus is related to the instability of coronary plaque. The C allele increases the risk of plaque vulnerability.