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[目的]明确阿维菌素、茚虫威及二者的复配制剂在稻田施用后,其有效成分阿维菌素B1a、阿维菌素B1b和茚虫威在稻田植株上的消解趋势、糙米中的残留水平。[方法]建立了高效液相色谱-三重串联质谱法HPLC-MS/MS(ESI+)同时检测稻田植株、稻田土壤、稻田水、稻壳以及糙米5种基质中阿维菌素与茚虫威残留量的分析方法。[结果]阿维菌素B1a和茚虫威均在0.002~1 mg/L范围内线性关系良好。二者在5种基质中0.01、0.02、0.2 mg/L添加水平的回收率分别为71.2%~102.0%、72.6%~106.3%,变异系数分别为1.4%~13.2%、2.6%~19.1%(n=5)。阿维菌素B1b在0.001~0.05 mg/L范围内线性关系良好,在0.000 4、0.004、0.04 mg/L三个添加水平的平均回收率为78.0%~101.7%,变异系数为2.6%~20.8%(n=5)。阿维菌素B1a、阿维菌素B1b和茚虫威的检出限分别为0.2、0.004、0.2μg/kg,定量限分别为0.01、0.000 4、0.01 mg/kg。方法的准确度、精密度满足农残检测的要求。采用该方法对施药后的水稻取样检测,144份糙米中阿维菌素与茚虫威的残留量均低于检出限。[结论]该方法简便、灵敏、节约成本,可用于水稻中农药多残留的快速检测。
[Objective] The aim of this study was to determine the trend of digestion of abamectin B1a, abamectin B1b and indoxacarb on rice plants after the combination of avermectin, indoxacarb and their combination in paddy field. Brown rice in the residual level. [Method] The simultaneous determination of abamectin and indoxacarb in five kinds of matrices of paddy field, paddy soil, paddy water, rice husk and brown rice by high performance liquid chromatography-triple tandem mass spectrometry was established by HPLC-MS / MS Quantitative analysis method. [Result] Avermectin B1a and indoxacarb all had good linearity in the range of 0.002-1 mg / L. The recoveries of the two compounds in the five substrates were 71.2% ~ 102.0%, 72.6% ~ 106.3%, and the coefficients of variation were 1.4% ~ 13.2%, 2.6% ~ 19.1% n = 5). Avermectin B1b had a good linearity in the range of 0.001-0.05 mg / L. The average recoveries of the three addition levels of 0.000 4,0.004 and 0.04 mg / L ranged from 78.0% to 101.7% with coefficients of variation from 2.6% to 20.8 % (n = 5). The detection limits of abamectin B1a, abamectin B1b and indoxacarb were 0.2,0.004 and 0.2 μg / kg, respectively. The limits of quantification were 0.01, 0.0004 and 0.01 mg / kg, respectively. Method accuracy, precision to meet the requirements of pesticide testing. With this method, the residues of abamectin and indoxacarb in 144 brown rice samples were all below the limit of detection. [Conclusion] The method was simple, sensitive and cost-effective, and could be used for rapid detection of pesticide residues in rice.