论文部分内容阅读
目的:采用N-甲基-N-硝基-N-亚硝基胍(MNNG)和40%乙醇灌胃建立大鼠慢性萎缩性胃炎(CAG)模型,同时采用破气苦降,饥饱失常和疲劳的方法进一步建立CAG脾气虚证大鼠模型。观察CAG脾气虚证大鼠不同时期病理形态学的改变。方法:以170μg/mL的MNNG10mL/kg灌胃,150μg/mL的MNNG溶液给大鼠自由饮用,40%乙醇,2mL/只,每周2次,在此基础上第5周采用破气苦降,饥饱失常和疲劳的方法建立CAG脾气虚证大鼠模型,连续10周。每2周随机处死CAG脾气虚证大鼠2只进行光镜观察。结果:随着造模时间的延长动物胃部病变的进程是从正常胃黏膜-慢性胃炎-萎缩性胃炎-肠上皮化生-不典型增生-胃癌前病变。结论:采用MNNG和40%乙醇灌胃以及破气苦降,饥饱失常和疲劳的方法可成功建立慢性萎缩性胃炎脾气虚证模型。
OBJECTIVE: To establish chronic atrophic gastritis (CAG) model in rats by intragastric administration of N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and 40% ethanol, And fatigue method to further establish CAG spleen qi deficiency rat model. To observe the pathological changes of CAG in rats with spleen-qi deficiency at different stages. Methods: Rats were drank freely with 40μg ethanol and 2ml MNNG at 170μg / mL and 150μg / ml MNNG solution twice daily. On the basis of this, , Hunger and malaria disorders and fatigue method to establish CAG spleen qi deficiency rat model for 10 weeks. Two rabbits were randomly sacrificed every 2 weeks to observe the light microscopy. Results: With the extension of modeling time, the process of gastric lesion in animals was from normal gastric mucosa - chronic gastritis - atrophic gastritis - intestinal metaplasia - dysplasia - precancerous lesions. Conclusion: The model of chronic atrophic gastritis with spleen-qi deficiency syndrome can be successfully established by intragastric administration of MNNG and 40% ethanol, as well as the method of dampness, bitterness, starvation and fatigue.