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目的探讨乙醇首过代谢发生的部位,从而为酒精性疾病的治疗提供新的思路。方法Wistar大鼠40只随机分成两组:模型组24只,对照组16只,建立大鼠酒精模型。用酶组织细胞化学染色技术,在光镜下观察肝胃乙醇脱氢酶(alcoholdehydrogenaseADH)活性变化,并用灰度图像分析仪进行半定量。结果长期摄入乙醇后肝ADH活性进行性增加,胃ADH活性进行性下降,两者对照差异显著(P<0.05)。结论结合文献得出乙醇的首过代谢发生在胃;诱导胃ADH的活性,可降低乙醇对机体的毒性作用。
Objective To investigate the site of first-pass metabolism of alcohol and to provide a new idea for the treatment of alcoholic diseases. Methods Forty Wistar rats were randomly divided into two groups: model group (n = 24) and control group (n = 16). Alcohol model was established in rats. The activity of alcohol dehydrogenase (ADH) in liver and stomach was observed under light microscopy with enzyme-linked immunosorbent assay. Semi-quantitative analysis was performed with gray image analyzer. Results After prolonged ingestion of ethanol, ADH activity in the liver increased progressively, and gastric ADH activity decreased progressively. The difference between the two groups was significant (P <0.05). Conclusions The literature shows that the first pass of ethanol occurs in the stomach and induces the activity of ADH in the stomach, which can reduce the toxic effect of ethanol on the body.