目的以C57BL/6J小鼠皮下接种B16黑色素瘤为模型,研究载有抗血管生成序列的逆转录病毒载体的基因治疗系统,对黑色素瘤的抗肿瘤效应及其对血液的近期影响。方法携带hRI的cDNA序列的重组转录病毒pLNCX-hRI颗粒被浓缩和纯化后,以空载体病毒颗粒及生理盐水为对照,静脉注射于肿瘤模型小鼠体内。检测目的基因在小鼠体内各脏器的表达水平,其对肿瘤生长的抑制作用,以及血液生化和细胞指标。结果转染后hRI基因的表达抑制了肿瘤生长,同时在观察期内无血液指标的改变。结论携带hRI基因的逆转录病毒载体基因治疗系统,可以在体内实现对B16黑色素瘤的抑制作用。 OBJECTIVE: To study the anti-tumor effect of anti-angiogenic retroviral vector gene therapy system and its immediate effect on blood in C57BL / 6J mice subcutaneously inoculated with B16 melanoma. Methods After the pLNCX-hRI particles carrying the hRI cDNA sequence were concentrated and purified, the empty vector virus particles and normal saline were used as control and injected intravenously into the tumor model mice. Detection of the target gene expression levels in various organs of mice, its inhibitory effect on tumor growth, and blood biochemical and cellular indicators. Results The expression of hRI gene inhibited the tumor growth after transfection, meanwhile there was no change of blood index during the observation period. Conclusion The retroviral vector gene therapy system carrying hRI gene can inhibit the B16 melanoma in vivo.