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以３０～４０日龄山羊的睾丸间质细胞为研究对象，采用体外细胞培养的方法，用胰岛素样生长因子－Ⅰ（ＩＧＦ－Ⅰ）和ｈＣＧ进行不同的处理，用放射免疫分析法（ＲＩＡ）测定了睾酮和ｃＡＭＰ的含量，用荧光分光光度分析法测定了ＤＮＡ含量。结果表明，ＩＧＦ－Ⅰ能促进体外培养的山羊睾丸间质细胞ｃＡＭＰ生成、睾酮分泌及ＤＮＡ合成，以上作用随ＩＧＦ－Ⅰ剂量的增大而加强，并且与ｈＣＧ有协同作用。根据以上结果首次提出ＩＧＦ－Ⅰ调节山羊睾丸间质细胞分泌睾酮机理的假设：ＩＧＦ－Ⅰ与其膜受体结合引起ｃＡＭＰ增多，ｃＡＭＰ通过激活一系列合成ＤＮＡ和睾酮所需酶类而使ＤＮＡ和睾酮的合成增加。这与催乳素（ＰＲＬ）和白细胞介素－１α（ＩＬ－１α）等含氮激素（因子）调节间质细胞分泌睾酮的机理不同，而与ＦＳＨ、ＬＨ等含氮激素调节性腺分泌类固醇激素的作用机理相似。
The 30-40 day-old goat Leydig cells were used as experimental objects. The cells were cultured in vitro and treated with insulin-like growth factor-I (IGF-Ⅰ) and hCG for different treatments. Radioimmunoassay (RIA) The contents of testosterone and cAMP were determined and the DNA content was determined by fluorescence spectrophotometry. The results showed that IGF-Ⅰcan promote cAMP production, testosterone secretion and DNA synthesis of goat testicular stromal cells cultured in vitro. The above effects were enhanced with the increase of IGF-Ⅰ dose, and synergistic with hCG. Based on the above results, the hypothesis that IGF-Ⅰ regulates the secretion of testosterone by goat testicular interstitial cells was first proposed: the binding of IGF-Ⅰ to its membrane receptors leads to the increase of cAMP, which activates DNA and testosterone by activating a series of enzymes required for synthesis of DNA and testosterone Increase in the synthesis. This is different from prolactin (PRL) and interleukin-1α (IL-1α) and other nitrogenous hormones (factors) regulation of interstitial cells to secrete testosterone different mechanism, and with FSH, LH and other nitrogen-containing hormones regulate gonadal secretion of steroid hormone The mechanism of action is similar.