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该项目以分子标志物为切入点,以高通量组学技术为基础,筛选出胃癌相关的分子标志物,对胃癌候选分子标志物进行生物学功能研究。结果发现,IPO-38、胃泌素、IL-33以及循环血中的FAM5C和MYLK高甲基化等胃癌血清诊断学标志物可作为胃癌早期诊断的标志物。研究还发现,FRZB、TXNL2、PHF10、MPS-1、PTP1B在胃癌组织中均呈高表达,与肿瘤细胞的增殖、分化、凋亡和侵袭等有关;胃癌细胞中AE1高表达可以将p16扣押在胞质,阻止其入核而起到原癌基因的作用;IRX1和miR-126在胃癌组织中均呈低表达,在胃癌中的作用类似抑癌基因。该研究为筛选胃癌候选标志物、开发诊断、分子靶点和预后判断等临床转化研究奠定了良好的基础,并荣获2008年国家科技进步二等奖和2012年上海市科技进步一等奖。
Based on high-throughput omics technology, the project screened out molecular markers related to gastric cancer and studied the biological function of molecular markers of gastric cancer. The results showed that serological diagnostic biomarkers such as IPO-38, gastrin, IL-33 and FAM5C and MYLK hypermethylation in circulating blood could be used as markers for early diagnosis of gastric cancer. The study also found that, FRZB, TXNL2, PHF10, MPS-1, PTP1B were highly expressed in gastric cancer tissue, and tumor cell proliferation, differentiation, apoptosis and invasion, etc .; gastric cancer cells AE1 overexpression p16 seizure in Cytoplasm, prevent it from entering the nucleus and play a proto-oncogene role; IRX1 and miR-126 were low expression in gastric cancer tissue, the role of gastric cancer in the tumor suppressor gene. This study lays a good foundation for the screening of gastric cancer candidate markers, developmental diagnosis, molecular targets and prognostic judgment, and won the second prize of National Science and Technology Progress Award in 2008 and the first prize of Shanghai Science and Technology Progress Award in 2012.