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目的研究启膈散对食管癌细胞微丝骨架的影响,并探讨其抑制转移的机制。方法采用高分化人食管癌细胞TE1、Eca109及低分化人食管癌细胞TE13,将细胞分为对照组(未加药)及启膈散(100μg/mL)组。应用免疫荧光法观察各组食管癌细胞形态的变化,激光共聚焦显微镜观察启膈散对食管癌细胞微丝排列的影响。采用细胞阻抗检测技术检测启膈散对食管癌细胞同基质黏附力及细胞迁移能力的影响。结果启膈散组食管癌细胞TE1、TE13及Eca109中微丝排列更规则,伪足消失,对照组细胞中微丝排列混乱,且以细胞膜微丝排列不规则更为明显,在细胞膜上微丝排列缺乏同向性,向外伸出伪足。与对照组比较,启膈散组食管癌细胞TE1和TE13中形态规则的细胞数目明显增多(P<0.05),TE1与TE13细胞经启膈散作用后细胞与基质的黏附力及迁移能力均明显降低(P<0.05,P<0.01)。结论启膈散能够抑制食管癌细胞发生微丝骨架重排,使食管癌细胞同基质之间的黏附力降低,从而抑制细胞的迁移。
Objective To study the effect of Qigansan on the microfilament cytoskeleton of esophageal cancer cells and to explore its mechanism of inhibiting metastasis. Methods The cells were divided into control group (untreated group) and Qige San (100 μg / mL) group by using highly differentiated human esophageal cancer cell lines TE1 and Eca109 and poorly differentiated human esophageal cancer cell line TE13. Immunofluorescence method was used to observe the morphological changes of esophageal cancer cells in each group. The effect of QG on the arrangement of microfilaments in esophageal cancer cells was observed with confocal microscopy. The effect of Qige Powder on the matrix adhesion and cell migration ability of esophageal cancer cells was detected by cell impedance assay. Results The microfilaments arranged in TE1, TE13 and Eca109 cells in Kaige powder group were more regular and pseudopodia disappeared. The microfilaments arranged in the control group were disordered, and the irregular arrangement of the microfilaments in the cell membrane was more obvious. Arrange the lack of coherence, outward protruding pseudo-foot. Compared with the control group, the number of regular cells in esophageal cancer cells TE1 and TE13 in Kaige powder group increased significantly (P <0.05), and the adhesion and migration ability of TE1 and TE13 cells to matrix were obvious Decreased (P <0.05, P <0.01). Conclusion Qige Powder can inhibit the rearrangement of actin cytoskeleton in esophageal cancer cells and decrease the adhesion of esophageal cancer cells to the matrix and thus inhibit the migration of esophageal cancer cells.