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为了开发利用吸水链霉菌BS-112菌株产生的抗真菌活性物质防治家蚕真菌病,在单因素试验基础上通过正交试验优化BS-112菌株分泌产生抗真菌活性物质的发酵培养基配方。优化后的最佳发酵培养基配方为:18.0g/L葡萄糖,12.0g/L玉米粉,35.0g/L黄豆饼粉,0.4g/LKH2PO4,0.8g/LMgSO4。优化发酵培养基配方后的菌株发酵液对家蚕病原绿僵菌的抗菌效价达到4916.85μg/mL,较基础培养基提高了3.08倍。分别以价格低廉的玉米粉和豆饼粉作为发酵培养基的缓效碳源和有机氮源,有利于该菌株在蚕用抗生素产业化开发的应用。
In order to develop antifungal active substances against streptomyces hygroscopicus BS-112 strain to prevent and cure silkworm mycosis, orthogonal test was used to optimize the fermentation medium formula of antifungal active substance secreted by BS-112 strain on the basis of single factor experiment. Optimized fermentation medium recipe: 18.0g / L glucose, 12.0g / L corn flour, 35.0g / L soybean meal, 0.4g / LKH2PO4, 0.8g / LMgSO4. The antibacterial titer of the strain fermentation broth on the pathogen Metarhizium anisopliae reached 4916.85μg / mL, which was 3.08 times higher than that of the basal medium. The low-cost corn flour and bean cake powder as the slow-acting carbon source and the organic nitrogen source of the fermentation medium are favorable for the industrial application of the antibiotic in the silkworm.