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应用传统及PCRDGGE方法(denaturinggradientgelelectrophoresis),分别对不同浓度乙草胺、甲胺磷胁迫下黑土中可培养真菌CFU(colonyformingunits)、种群丰富度(richness)及种群结构动态变化规律进行了研究.结果表明,在实验室微域条件下,乙草胺对黑土可培养真菌CFU的影响随处理浓度的增加而抑制作用增强,表现出由低浓度(50mg·kg-1)时的刺激生长到高浓度(250mg·kg-1)时的长期抑制效应;250mg·kg-1甲胺磷在8周处理过程中对土壤可培养真菌生长具有显著的刺激效应,使可培养真菌CFU比对照增加10倍,但50和150mg·kg-1甲胺磷处理对土壤可培养真菌CFU无显著影响.种群丰富度系数分析结果表明,高、中浓度乙草胺处理可使土壤可培养真菌种群丰富度不可逆地降低.土壤真菌rDNA特异PCRDGGE聚类分析结果表明,不同浓度乙草胺、甲胺磷处理均不同程度地对土壤可培养真菌的种群组成和结构造成影响,其中甲胺磷尤为显著.
The dynamic changes of colonyformingunits (CFU), richness and population structure of CEFs cultured in black soil with different concentrations of acetochlor and methamidophos were studied by traditional and PCR-DGGE methods, respectively. The results showed that , The effect of acetochlor on CFU of culturable fungi in black soil was enhanced with the increase of the treated concentration under laboratory microdomains, which showed that the inhibitory effect of acetochlor on CFU increased from low concentration (50mg · kg-1) to high concentration ( 250mg · kg-1). The methamidophos at 250mg · kg-1 significantly stimulated the growth of soil-culturable fungi during 8 weeks of treatment, resulting in a 10-fold increase in CFU of culturable fungi compared with the control 50 and 150 mg · kg -1 methamidophos had no significant effect on CFU of soil culturable fungi.The analysis of population richness coefficient showed that high and medium concentrations of acetochlor treatment could irreversibly reduce the population richness of soil culturable fungi. The result of rDNA-specific PCRDGGE cluster analysis of soil fungi showed that acetochlor and methamidophos at different concentrations affected the population composition and structure of soil culturable fungi to varying degrees. Methamidophos is particularly significant.