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为探讨抗原呈递细胞 (APCs)在软下疳发病机制中的作用 ,将树突状细胞 (DCs)和巨噬细胞 (MQs)与流感嗜血杆菌、杜克雷嗜血杆菌及其纯化抗原脂寡糖 (LOS)、细胞致死性肿胀毒素 (HdCDT)共育 ,测定它们的吞噬活性及6、2 4h时前炎症细胞因子TNF α、IL 1 β、IL 6、IL 8的分泌情况 ;将抗原作用后的细胞与自体CD4 + T淋巴细胞共育 ,测定T淋巴细胞增生及 48h细胞上清液中的细胞因子IFN γ、IL 4、IL 1 3的水平。结果 :约 6%～ 2 7%的DCs和 1 4%～ 2 6%的MQs吞噬杜克雷嗜血杆菌的不同菌株及无荚膜流感嗜血杆菌、大肠杆菌。杜克雷菌的不同菌株诱导DCs、MQs分泌大量的TNF α、IL 6、IL 8(P <0 .0 5 ) ;LOS诱导的前炎症细胞因子水平比细菌低 5 0 %～ 67% ;HdCDT不诱导任何前炎症细胞因子的产生。 2种细菌作用后的DCs、MQs能激活T淋巴细胞产生高水平IFN γ ,并诱导产生相似的T淋巴细胞增生 ,HdCDT无此作用。提示 :杜克雷菌能影响APCs活化T细胞的能力 ,并有助于Th1型反应。
In order to investigate the role of antigen-presenting cells (APCs) in the pathogenesis of infantile hyssopium, dendritic cells (DCs) and macrophages (MQs) were incubated with Haemophilus influenzae, Haemophilus ducreyi and their purified antigen lipooligosaccharides LOS and HdCDT, and their phagocytic activity and secretion of proinflammatory cytokines TNFα, IL 1 β, IL 6 and IL 8 at 6 and 24 h were measured. After antigen-induced The cells were co-cultured with autologous CD4 + T lymphocytes, and the levels of cytokines IFN-γ, IL-4 and IL-13 in T-lymphocyte proliferation and 48h cell supernatant were measured. Results: About 6% ~ 27% of DCs and 14% ~ 26% of MQs were swallowed by different strains of Haemophilus ducreyi and non-capsulatus influenzae and Escherichia coli. DCs were induced by different strains of Dukes Klebsiella, and MQs secreted a large amount of TNFα, IL-6 and IL-8 (P <0.05); LOS-induced proinflammatory cytokines levels were 50% -67% lower than those of bacteria; HdCDT did not induce Production of any pro-inflammatory cytokines. After two kinds of bacteria act on DCs, MQs can activate T lymphocytes to produce high level of IFNγ, and induce similar T lymphocyte proliferation without HdCDT. Tip: Du Klebs can affect the ability of APCs to activate T cells and contribute to Th1-type responses.