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利用逆转录PCR技术克隆了中国株HCV的核心蛋白基因和非结构3区蛋白基因,DNA序列无分析显示克隆的基因片段属HCV-Ⅰ型。将二个基因片段分别克隆至质粒表达载体内,构建了多株表达融合和非融合核心蛋白的工程菌及表达非结构3区蛋白的工程菌。建立了二种重组蛋白的纯化方法,
The core and non-structural 3-region genes of HCV in China were cloned by RT-PCR. No DNA sequence analysis showed that the cloned gene fragment was of HCV-Ⅰ type. Two gene fragments were cloned into the plasmid expression vector respectively, and several engineered bacteria expressing fusion and non-fusion core proteins and engineered bacteria expressing the non-structural 3-region protein were constructed. Established two kinds of recombinant protein purification methods,