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目的建立测定通乐颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的方法。方法采用HPLC法,色谱柱为Phenomenex C18柱,检测波长为320 nm,流动相为乙腈-水(20∶80),体积流量为1.0 mL/min。结果 2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在10~100μg/mL与峰面积呈良好线性关系(r=0.999 7),平均回收率为99.74%。结论本方法准确、简便、重复性好,可用于通乐颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定。
Objective To establish a method for the determination of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside in Tongle Granules. Methods The HPLC method was used on a Phenomenex C18 column with a detection wavelength of 320 nm. The mobile phase consisted of acetonitrile-water (20:80) and the volume flow rate was 1.0 mL / min. Results The linear range of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside was 10-100 μg / mL and the peak area was linear (r = 0.999 7) 99.74%. Conclusion The method is accurate, simple and reproducible. It can be used to determine 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside in Tongle Granule.