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目的建立绝经后骨质疏松症山羊模型并探讨其意义。方法将10只6～8岁雌性健康山羊,随机分为两组,假手术组(SHAM组,2只,开腹后缝合切口)和双侧卵巢切除术组(OVX组,8只,切除山羊的双侧卵巢),术前和术后4个月,分别自颈静脉抽血检测血生化指标,使用双能X线吸收法(DEXA)测量L1～L4的骨密度(BMD,g/cm2),并取椎体内松质骨做病理组织学观察。结果OVX手术及假手术均较顺利,无动物死亡;腹腔切除物经组织学确认为卵巢组织,OVX术后4个月椎体内松质骨与术前相比,骨小梁变细、数量轻度减少,间距增宽,骨髓腔扩大;OVX术后4个月碱性磷酸酶为(472±63)IU/L,其与BMD均较术前明显降低(P<0.05)。结论中老年雌性山羊可能是一种有效且经济的绝经后骨质疏松症大动物模型,对于骨质疏松性骨折内植物的基础研究具有广阔而深远的意义。
Objective To establish a model of postmenopausal osteoporosis goat and to explore its significance. Methods Ten healthy goats aged 6-8 years old were randomly divided into two groups: sham operation group (SHAM group, two open suture incision) and bilateral ovariectomy group (OVX group, eight goats Of bilateral ovaries). Blood biochemical indexes were measured from the jugular vein before and 4 months after operation. The bone mineral density (BMD, g / cm2) of L1 ~ L4 was measured by dual energy X-ray absorptiometry (DEXA) , And take cancellous bone in vertebral body for histopathological observation. Results OVX surgery and sham operation were smooth and no animals died. The peritoneal resection was confirmed by histology as ovarian tissue. Compared with preoperative, the trabecular bone became thinner at 4 months after OVX. Mild decrease, widening of interval and enlargement of bone marrow cavity. The alkaline phosphatase in the 4 months after OVX was (472 ± 63) IU / L, which was significantly lower than that of preoperative BMD (P <0.05). Conclusion Middle-aged and old female goats may be an effective and economical large animal model of postmenopausal osteoporosis, which has broad and far-reaching significance for the basic research of implants in osteoporotic fractures.