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为了从生理生化角度上揭示烟草花叶病毒(TMV)的抗病机理,以12个晒烟品种为试材,进行了人工接种鉴定试验,且选择具有代表性的免疫(延晒十号)、抗病(坝林晒烟)、中感(延晒四号)品种为试材,研究了接种前后的保护酶活性、MDA含量、叶绿素含量等生理生化指标变化规律。试验结果表明:在SOD、POD和CAT活性方面,随着接种时间的推进,3个对照处理的酶活性均逐渐上升,而3个接种处理则均呈先上升后下降的趋势;接种后6~24 d,3个品种接种处理的酶活性均比对照处理高,增加幅度最大的是延晒十号(免疫品种),最小的是延晒四号(中感品种);接种后36 d,3个接种处理均低于对照,衰减幅度由小到大依次为延晒十号、坝林晒烟、延晒四号。从MDA含量来看,所有处理均随接种时间延长逐渐增加,同一品种接种处理均高于对照处理,增加幅度最大的是延晒四号,最小的是延晒十号。从实际光合效率Y(Ⅱ)和叶绿素含量来看,与对照相比,3个接种处理的指标均下降,衰减幅度由小到大依次为延晒十号、坝林晒烟、延晒四号。上述指标在植物抗病反应中呈现规律性变化,从生理生化层面上反映了晒烟的TMV抗病机理。
In order to reveal the disease resistance mechanism of Tobacco Mosaic Virus (TMV) from physiological and biochemical aspects, artificial inoculation tests were conducted using 12 sun-cured tobacco cultivars, and a representative immunization (Yanzhao No.10) The results showed that there was no significant difference in the content of MDA and chlorophyll content between the two cultivars. The changes of protective enzyme activity, MDA content, chlorophyll content and other physiological and biochemical indexes before and after inoculation were studied. The results showed that the activities of SOD, POD and CAT increased gradually with the time of inoculation in three control treatments, while the three inoculation treatments showed the trend of first increasing and then decreasing. After 6 ~ On the 24th day, the activity of inoculation of three varieties was higher than that of the control. The most significant increase was Yanyan No. 10 (immune variety), the smallest was Yanan No. 4 (susceptible variety); 36 d after inoculation A vaccination were lower than the control, the attenuation range from small to large, followed by the sun on the 10th, dam forest sun, Yan on the 4th. From the MDA content point of view, all the treatments are gradually increased with the extension of inoculation time, the same species inoculation were higher than the control treatment, the largest increase is Yan 4, the smallest is Yan 10. From the actual photosynthetic efficiency of Y (Ⅱ) and chlorophyll content point of view, compared with the control, the three indicators of inoculation were decreased, the attenuation range from small to large, followed by the sun on the 10th, dam forest sun, on the 4th . The above indexes showed regular changes in the disease resistance of plants, and reflected TMV disease resistance mechanism of sun-cured tobacco from the physiological and biochemical aspects.