论文部分内容阅读
目的 :监测SARS患者血清丙二醛 (MDA)和一氧化氮 (NO)、诱导型一氧化氮合酶 (iNOS)浓度并探讨其意义。方法 :采用硫代巴比妥酸比色法测定MDA、硝酸还原酶法测定NO、酶联免疫吸附法定量测定iNOS ,检测早期、恢复期SARS患者以及出院后SARS随访者 ,一线未患SARS医护人员 ,健康体检者血清中MDA、NO和iNOS含量。结果 :SARS早期血清MDA含量显著高于恢复期 (P <0 0 1) ,恢复期均高于出院后随访时和一线医护人员与体检者(P <0 0 1)。SARS早期组NO水平高于其他各组 (P <0 0 5 ) ,恢复期组、随访组均与一线医护人员组和健康对照组有显著差异 (P <0 0 1)。SARS早期iNOS含量高于其他各期 (P <0 0 1) ,恢复期与一线医护人员有显著差异 (P <0 0 5 ) ,其余组间两两比较均无显著差异 (P >0 0 5 )。结论 :SARS的发病机制中病理损伤与自由基有关。
OBJECTIVE: To monitor serum concentrations of malondialdehyde (MDA), nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in SARS patients and explore its significance. Methods: MDA was detected by thiobarbituric acid colorimetric method, NO was measured by nitrate reductase method, iNOS was quantified by enzyme-linked immunosorbent assay (ELISA), SARS patients in early and convalescent phase and SARS follow-up were discharged. Serum levels of MDA, NO and iNOS in health staff and health examination personnel. Results: The serum levels of MDA in the early stage of SARS were significantly higher than those in the recovery stage (P <0.01), and the recovering stage was higher than that in the first-line medical staff and the physical examination after discharge from the hospital (P <0.01). The levels of NO in the early stage of SARS were higher than those in other groups (P <0 05). There was significant difference between the recovery group and the follow-up group and the first-line medical staff and the healthy control group (P <0.01). The content of iNOS in early stage of SARS was higher than that in other stages (P <0.01), and there was a significant difference (P <0 05) between the recovery period and the first-line medical staff. There was no significant difference between the other groups (P> 0.05). ). Conclusion: Pathological injury in pathogenesis of SARS is related to free radicals.