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目的:探讨甲醛对小鼠血液中抗氧化酶及微量元素的影响。方法:选取80只健康昆明小鼠(由北华大学实验动物中心提供),体重18~24g,雌雄各半。将小鼠随机分4组,即对照组、低剂量组(1/40LD50)、中剂量组(1/20LD50)、高剂量组(1/10LD50),每组20只,每日定时静式吸取2h,连续6周,对照组不接触甲醛,其他处置均与感染组相同:6周后小鼠断头取血1mL,与微波消解器中加入纯硝酸消解2min,定容至10mL,原子吸收法测定Zn、Mn、Fe含量,同时取出0.02mL全血,配成1mL溶血液,采用南京建成生物制品厂提供的测试盒测定GSH-Px活性、MDA的含量和总抗氧化能力。结果:甲醛染毒小鼠全血脂质过氧化变化分析中,与对照组比较,高剂量组小鼠GSH-Px的活性显著降低,中、高剂量组小鼠总抗氧化能力显著降低,而中、高剂量组MDA含量显著升高(P<0.05);甲醛染毒全血微量元素Zn、Mn、Fe的变化分析中,与对照组比较,染毒6周中、高剂量组小鼠全血微量元素Zn、Fe明显降低(P<0.05),高剂量组Mn含量明显降低(P<0.05)。结论:甲醛可损伤抗氧化酶系统,使GSH-Px、总抗氧能力降低,MDA含量升高,全血微量元素Fe、Zn和Mn也显著降低,为探讨甲醛对机体健康影响的作用机制提供的线索。
Objective: To investigate the effect of formaldehyde on antioxidant enzymes and trace elements in blood of mice. Methods: Eighty healthy Kunming mice (provided by Experimental Animal Center of Beihua University) were selected and weighed 18 ~ 24g. The mice were randomly divided into 4 groups: control group, low dose group (1 / 40LD50), middle dose group (1 / 20LD50) and high dose group (1 / 10LD50) 2h, 6 consecutive weeks, the control group did not touch formaldehyde, the other treatment were the same as the infected group: 6 weeks after the mice decapitated blood 1mL, microwave digestion with pure nitric acid digestion 2min, constant volume to 10mL, atomic absorption The contents of Zn, Mn and Fe were determined. At the same time, 0.02 mL of whole blood was taken out and dissolved in 1 mL of hemolytic solution. The activity of GSH-Px, the content of MDA and the total antioxidant capacity were measured by using the test box provided by Nanjing Jiancheng Biological Products Factory. Results: Compared with the control group, the GSH-Px activity of mice in the high-dose group was significantly decreased, while the total antioxidant capacity of the mice in the medium-dose and high-dose groups was significantly decreased The content of MDA in medium and high dose groups was significantly increased (P <0.05). Compared with the control group, in the changes of trace elements Zn, Mn and Fe in whole blood of formaldehyde exposure, The contents of trace elements Zn and Fe in blood decreased significantly (P <0.05), and the content of Mn in high dose group decreased significantly (P <0.05). CONCLUSION: Formaldehyde can damage the antioxidant enzyme system and decrease GSH-Px, total antioxidant capacity and MDA content, as well as trace elements Fe, Zn and Mn in whole blood. In order to explore the mechanism of action of formaldehyde on body health The clues.