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探讨一氧化氮合酶（ｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ＮＯＳ）和内皮素（ｅｎｄｏｔｈｅｌｉｎ，ＥＴ）及ＥＴ转换酶（ｅｎｄｏｔｈｅｌｉｎｃｏｎｖｅｒｔｉｎｇｅｎｚｙｍｅ，ＥＣＥ）等在受照射肺中的基因表达变化，揭示其在肺纤维化发生中的参与作用。方法用６０Ｃｏ对大鼠肺进行３０Ｇｙ照射，照后４，８，２４天提取总ＲＮＡ，分别做ＲＴ－ＰＣＲ；对照射后１，３，５个月肺组织进行ＥＴ肽的Ｗｅｓｔｅｒｎｂｌｏｔ和ＮＯＳ－ｍＲＮＡ原位杂交。结果照射后肺ＮＯＳｍＲＮＡ转录减弱，ＥＴｍＲＮＡ增强，ＥＣＥｍＲＮＡ在第８和２４天增强，ＥＴＢ－ＲｍＲＮＡ在第２４天转为阴性。照射后１，３，５月肺组织ＥＴ肽合成增加。照射后５个月ＮＯＳｍＲＮＡ原位杂交呈阴性。结论ＥＴ和ＮＯ之间平衡失调可能与肺纤维化发生有关。
To investigate the changes of gene expression of nitric oxide synthase (NOS), endothelin (ET) and endothelinconvertingenzyme (ECE) in irradiated lungs, and to explore its role in the pathogenesis of pulmonary fibrosis . Methods The lungs of rats were irradiated with 30Gy at 60Co, and the total RNA was extracted at 4, 8 and 24 days after irradiation. RT-PCR was performed respectively. Western blot and NOS-mRNA of ET peptides were performed on the lungs at 1, 3 and 5 months after irradiation In situ hybridization. Results After irradiation, the expression of NOS mRNA decreased, ET mRNA increased, ECE mRNA increased on day 8 and 24, and ETB-R mRNA turned negative on the 24th day. 1, 3, 5 months after irradiation, ET peptide synthesis in lung tissue increased. NOS mRNA in situ hybridization was negative 5 months after irradiation. Conclusion The imbalance between ET and NO may be related to the occurrence of pulmonary fibrosis.