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目的建立反相高效液相色谱法测定大红口服液中芦荟大黄素含量的方法。采用Kromasil C18反相色谱柱(5μm250mm×4.6mm),流动相为甲醇一水(75:25);流速为1.0mL.min-1。检测波长254nm。芦荟大黄素在9.135~21.315μg/mL范围内呈良好的线性关系(r=0.9998)。芦荟大黄素平均回收率分别为100.25%(RSD=0.89)。结论本法简便、准确,可用于大红口服液中芦荟大黄素的含量测定。
Objective To establish a method for the determination of aloe-emodin in Dahong Oral Liquid by reversed-phase high performance liquid chromatography. A Kromasil C18 reversed-phase column (5 μm 250 mm × 4.6 mm) was used with a mobile phase of methanol-water (75:25) and a flow rate of 1.0 mL · min -1. Detection wavelength 254nm. Aloe-emodin in the range of 9.135 ~ 21.315μg / mL showed a good linear relationship (r = 0.9998). The average recoveries of aloe-emodin were 100.25% (RSD = 0.89). Conclusion This method is simple and accurate and can be used to determine the content of aloe-emodin in Dahong Oral Liquid.