论文部分内容阅读
目的比较ＭＰＴＰ处理的小鼠与６－ＯＨＤＡ损毁大鼠ＰＤ模型的病理变化。方法应用免疫组化方法观察两种ＰＤ模型的中脑腹侧多巴胺能神经元，星形胶质细胞的变化。结果免疫组化方法结果表明：Ｃ５７ＢＬ小鼠在ＭＰＴＰ处理后，脑内酪氨酸羟化酶阳性神经元的数目从第４ｄ开始有所减少；黑质区域的ＧＦＡＰ免疫阳性星形胶质细胞数目从第１ｄ起即有增加。大鼠ＰＤ模型较早出现损毁侧ＴＨ免疫阳性神经元明显减少的现象，损毁２月后几乎完全消失；而在黑质区的ＧＦＡＰ免疫阳性星形胶质细胞数目增多现象出现较晚。结论两种动物模型从不同侧面反映了帕金森病的病理特征，６－ＯＨＤＡ损毁中脑ＤＡ能神经元制成的大鼠ＰＤ模型比ＭＰＴＰ处理的Ｃ５７ＢＬ小鼠ＰＤ模型更接近反映ＰＤ的病理变化。
Objective To compare the pathological changes of MPTP-treated and 6-OHDA-injured PD rats. Methods The changes of ventral dopaminergic neurons and astrocytes in the midbrain of the two PD models were observed by immunohistochemistry. Results The results of immunohistochemistry showed that the number of tyrosine hydroxylase positive neurons in brain of C57BL mice decreased from the 4th day after MPTP treatment. The number of GFAP immunopositive astrocytes in the substantia nigra region From the first day has increased. The rat model of PD appeared earlier and the number of damaged TH-immunoreactive neurons decreased significantly. The damage disappeared almost completely after February, while the number of GFAP immunopositive astrocytes in the substantia nigra was increased later. Conclusions The two animal models reflect the pathological features of Parkinson’s disease from different aspects. The rat PD model of 6-OHDA-damaged DA neurons in the midbrain is more similar to the pathological changes of PD than the MPTP-treated C57BL mouse PD model .