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目的检测人肝癌高转移细胞系生长抑素受体mRNA和蛋白的表达,探讨生长抑素对肝癌细胞株增殖的影响及其作用机制。方法应用免疫组化和RT-PCR的方法检测人肝癌细胞系中五种生长抑素受体亚型的表达,并进行半定量分析;应用MTT法检测8肽生长抑素对肿瘤细胞增殖的影响。结果肝癌细胞HCCLM3中存在SSTR1-5表达。生长抑素抑制肝癌细胞株HCCLM3的增殖并呈浓度依赖性,其作用可以被PTp抑制剂正钒酸钠所抑制;HCCLM3细胞表达5种生长抑素受体的mRNA和蛋白,表达强度由强到弱依次为SSTR2、SSTR1、SSTR4、SSTR3、SSTR5。SSTR2和 SSTR1的表达强度明显强于SSTR3、SSTR4、SSTR5(P<0．05),应用生长抑素后受体亚型SSTR2、 SSTR3、SSTR5 mRNA表达强度明显高于SSTR1、4 mRNA(P<0．05)。结论五种生长抑素受体亚型均存在于HCCLM3肝癌细胞系中,生长抑素可以明显抑制肝癌细胞的增殖。SSTR2 SSTR3、SSTR5和生长抑素结合后通过激活PTP抑制肿瘤细胞的生长和增殖。
Objective To detect the expression of somatostatin receptor mRNA and protein in highly metastatic human hepatoma cell line and to investigate the effect of somatostatin on the proliferation of hepatoma cell line and its mechanism. Methods The expression of five somatostatin receptor subtypes in human hepatocellular carcinoma cell lines was detected by immunohistochemistry and RT-PCR and semi-quantitative analysis was performed. The effect of somatostatin 8 on the proliferation of tumor cells was detected by MTT assay . Results There was SSTR1-5 expression in HCCLM3 cells. Somatostatin inhibited the proliferation of hepatocellular carcinoma cell line HCCLM3 in a concentration-dependent manner, and its inhibitory effect was inhibited by sodium orthovanadate (PTP inhibitor). HCCLM3 cells expressed mRNA and protein of five somatostatin receptors, The weak order is SSTR2, SSTR1, SSTR4, SSTR3, SSTR5. The expression of SSTR2, SSTR3 and SSTR5 mRNA in SSTR2, SSTR3 and SSTR5 was significantly higher than that in SSTR1, SSTR4 and SSTR5 (P <0.05) .05). Conclusion All five somatostatin receptor subtypes are present in HCCLM3 hepatocellular carcinoma cell lines. Somatostatin can significantly inhibit the proliferation of hepatoma cells. SSTR2 SSTR3, SSTR5 and somatostatin inhibit tumor cell growth and proliferation by activating PTP.