论文部分内容阅读
目的：研究四氢小檗碱（ＴＨＢ）对大鼠全脑缺血再灌注损伤的作用。方法：采用改良的４血管结扎法制作大鼠全脑缺血再灌注损伤模型，比较ＴＨＢ组和对照组的脑组织超氧化物歧化酶（ＳＯＤ）活性和丙二醛（ＭＤＡ）含量及细胞形态学改变。结果：ＴＨＢ（缺血前后分次ｉｐ４０、４０、２０ｍｇ／ｋｇ）能提高全脑缺血３０ｍｉｎ再灌注３０ｍｉｎ大鼠大脑皮质和海马ＳＯＤ活性，并降低ＭＤＡ含量。延长再灌注时间至７２ｈ，大鼠大脑皮质、纹状体和海马神经元形态学受损较重，ＴＨＢ（ｉＰ４０ｍｇ／ｋｇ·ｄ×４ｄ）有明显保护大脑皮质和纹状体神经元的作用，但对海马组织无保护作用。结论：ＴＨＢ对短暂全脑缺血损伤有一定的保护作用。
Objective: To investigate the effect of tetrahydrobiopterine (THB) on global cerebral ischemia-reperfusion injury in rats. Methods: A rat model of global cerebral ischemia-reperfusion injury was established by modified 4-vessel ligation. Compared with the control group and THB group, the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and cell morphology Learning to change. Results: THB (ip40, 40 and 20 mg / kg) could increase SOD activity and decrease MDA content in cerebral cortex and hippocampus of rats after 30 min of reperfusion for 30 min after global cerebral ischemia. Prolonging the reperfusion time to 72h, the morphological damage of cerebral cortex, striatum and hippocampus neurons in rats was severe. THB (iP40mg / kg · d × 4d) significantly protected the neurons of cerebral cortex and striatum, But no protective effect on hippocampal tissue. Conclusion: THB has a protective effect on transient global cerebral ischemia.