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目的研究肉桂醛对高糖诱导心肌成纤维细胞增殖,Ⅰ、Ⅲ型胶原蛋白分泌的影响及细胞外信号调节激酶1/2(ERK1/2)信号通路的作用。方法体外培养新生大鼠心肌成纤维细胞,MTT检测肉桂醛对高糖环境下细胞增殖的影响;ELISA检测Ⅰ、Ⅲ型胶原蛋白表达;RT-PCR检测Ⅰ、Ⅲ型胶原蛋白mRNA的表达;Western blot检测ERK1/2、p-ERK1/2的蛋白表达。结果与对照组比较,高糖组成纤维细胞增殖及Ⅰ、Ⅲ胶原蛋白表达增加(P<0.05)。与高糖组比较,肉桂醛组心肌成纤维细胞磷酸化ERK1/2蛋白表达降低[(3.14±0.12)vs(2.01±0.08),P<0.05)]。结论肉桂醛能抑制高糖诱导的心肌成纤维细胞增殖及Ⅰ、Ⅲ型胶原蛋白表达的增加,其作用可能与下调ERK1/2活性有关。
Objective To investigate the effect of cinnamaldehyde on the proliferation of cardiac fibroblasts induced by high glucose, the type Ⅰ and type Ⅲ collagen secretion and the signal transduction pathway of extracellular signal-regulated kinase 1/2 (ERK1 / 2). Methods The cultured neonatal rat cardiac fibroblasts were cultured in vitro. The effects of cinnamaldehyde on the proliferation of cells were detected by MTT assay. The expressions of collagen type Ⅰ and Ⅲ were detected by ELISA. The expressions of collagen type Ⅰ and Ⅲ were detected by RT-PCR. blot detection of ERK1 / 2, p-ERK1 / 2 protein expression. Results Compared with the control group, the proliferation of high glucose group fibroblasts and the expression of collagen Ⅰ, Ⅲ increased (P <0.05). Compared with high glucose group, the expression of phosphorylated ERK1 / 2 in cinnamaldehyde group decreased (3.14 ± 0.12 vs 2.01 ± 0.08, P <0.05). Conclusion Cinnamaldehyde can inhibit the proliferation of cardiac fibroblasts induced by high glucose and the increase of collagen type Ⅰ and Ⅲ, which may be related to the down-regulation of ERK1 / 2 activity.