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目的 建立一种简单的体外培养视网膜神经节细胞的方法。方法 采用Thy1 1单克隆抗体免疫吸附得到纯化的视网膜神经节细胞 ,将其接种于纯化培养的视网膜M櫣ｌｌｅｒ细胞上 ,在无血清培养液中共同培养。结果 视网膜神经节细胞在接种后 1d即可生长出短小的突起 ,至第 5d突起不断增长并可长出二级分支。结论 M櫣ｌｌｅｒ细胞在无血清培养液中 ,对体外培养的神经节细胞具有很好的支持营养作用。
Objective To establish a simple method of culturing retinal ganglion cells in vitro. Methods Purified retinal ganglion cells were obtained by immunosorbent assay with Thy1 1 monoclonal antibody. The cultured retinal ganglion cells were inoculated on purified retinal Müller cells and co-cultured in serum-free medium. Results The retinal ganglion cells grew short protuberances 1 day after inoculation, and the neurite outgrowth increased on day 5d and secondary branches could grow. Conclusion M 櫣 ller cells in serum-free medium, in vitro cultured ganglion cells have a good support for nutrition.