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目的研究大鼠创伤性脑水肿皮质中AQP-4与MMP-9的表达关系及甲强龙对其表达的影响。方法采用成年雄性SD大鼠随机分为甲强龙组(n=25)、模型组(n=25)、假手术组(n=5);甲强龙组和模型组均采用改进Feeney法制作脑损伤模型。甲强龙组致伤后即刻腹腔内给药,模型组则注射对应生理盐水。根据处死时间分为6h、24h、3d、5d、7d,5组。过度麻醉并断头取脑,对损伤皮层采用HE染色及免疫组化进行AQP4与MMP-9的检测。结果在模型组与甲强龙组中6h已可见AQP4与MMP-9的表达,MMP-9与AQP-4的表达均在3d达到高峰,模型组AQP4及MMP-9的表达显著高于甲强龙组(P<0.05),相关系数(r=0.753,P<0.01)。结论创伤性脑损伤后大鼠AQP4与MMP9的表达呈正相关;甲强龙冲击治疗能够通过下调AQP4及MMP9的表达来发挥减轻脑水肿的作用。
Objective To investigate the expression of AQP-4 and MMP-9 in the cortex of traumatic cerebral edema in rats and the effect of methylprednisolone on its expression. Methods Adult male Sprague-Dawley rats were randomly divided into two groups: metforone group (n = 25), model group (n = 25) and sham operation group (n = 5) Brain injury model. The methylprednisolone group was administered intraperitoneally immediately after injury and the model group was injected with saline. According to the time of death is divided into 6h, 24h, 3d, 5d, 7d, 5 groups. Excessive anesthesia and decapitation of the brain, the injured cortex using HE staining and immunohistochemical detection of AQP4 and MMP-9. Results The expressions of AQP4 and MMP-9 were found in model group and methylprednisolone group at 6h, the expression of MMP-9 and AQP-4 peaked at 3d, and the expression of AQP4 and MMP-9 in model group was significantly higher than that in A (P <0.05), the correlation coefficient (r = 0.753, P <0.01). Conclusion The expression of AQP4 and MMP9 in rats after traumatic brain injury is positively correlated. Methylprednisolone treatment can relieve cerebral edema by down-regulating the expression of AQP4 and MMP9.