随着 PCR及其相关技术的不断发展和完善 ,其应用范围日益广泛 ,如何方便、快捷和高效地克隆PCR产物越来越成为众多研究者关注的焦点。克隆PCR产物有若干好处 ,一是有利于测序 ,二是方便产物的大量制备 ,从而满足利用其作为探针进行各种杂交反应及功能研究的需求。目前 ,T- A克 With the continuous development and improvement of PCR and related technologies, its application is becoming more and more widespread. How to clone PCR products easily, quickly and efficiently has become the focus of many researchers. There are several advantages to cloning PCR products, one is that it is beneficial to sequencing, and the second is to facilitate the preparation of a large number of products, so as to meet the needs of using them as probes for various hybridization reactions and functional studies. Currently, T-A g