我们采用激光光声光谱法和吸收光谱实时监测在不同的入射激光能量下,漆酶及铜兰蛋白的变化。研究了0.1mJ～9.6mJ/脉冲入射激光能量下,漆酶和铜兰蛋白的光声信号强度变化。 蛋白质分子都是由氨基酸首尾相接而成的共价多肽链组成,形成高聚体,具有一定的构象。维持蛋白质分子构象的化学键有:氢键、疏水键、范德华引力、离子键、二硫键、配位键。这种多种弱化 We used laser photoacoustic spectroscopy and absorption spectroscopy to monitor the changes of laccase and ceruloplasmin at different incident laser energies in real time. The changes of photoacoustic signal intensity of laccase and cerulein were studied under laser energy of 0.1mJ ~ 9.6mJ / pulse. Protein molecules are composed of amino acids linked together from the end of the covalent polypeptide chain, forming a high polymer, with a certain conformation. The chemical bonds that maintain the conformation of the protein molecule are: hydrogen bond, hydrophobic bond, van der Waals attraction, ionic bond, disulfide bond, coordination bond. This variety of weakening