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1993~ 1998年 ,构建了携有抗稻白叶枯病新基因 Xa2 3的近等基因系 ,命名为 CBB2 3。以全生育期高度感病并具有改良株型的籼稻品种 JG30为轮回亲本 ,与携有 Xa2 3的抗性供体 H4杂交 ,JG30 / H4的 F1 通过回交、自交直至 B5F4,各世代均用与目标基因 Xa2 3相对应的专化小种菌系 P6人工接种鉴定 ,同步进行株型和农艺性状选择 ,直至抗性稳定和农艺性状类似其轮回亲本。比较了 CBB2 3(携 Xa2 3)和 IRBB2 1(携 Xa2 1)对 2 0个菌系包括 10个菲律宾小种、3个日本小种和7个中国病原型代表菌系的抗谱 ,Xa2 3抗所有 2 0个菌系 ;Xa2 1抗 19个菌系 ,对菲律宾 10号小种则高度感病。用近等基因系 CBB2 3构建了 JG30 / CBB2 3组合的 F2 分离群体 ,通过 SSR标记筛选 ,初步将 Xa2 3定位于水稻第 11染色体。进一步筛选出 3个与 Xa2 3更紧密连锁的 AFL P标记。其中的 APKj2 3与 Xa2 3之间的图距约为 1.0 c M。并报道了在抗病育种中已有效的应用了携有 Xa2 3的近等基因系 CBB2 3。
From 1993 to 1998, a near isogenic line carrying the new gene Xa2 3 against rice bacterial leaf blight was constructed and named CBB2 3. The indica variety JG30 with highly susceptible and improved plant type during the whole growth period was the reincarnated parent and crossed with the resistant donor H4 carrying Xa2 3. The F1 of JG30 / H4 was crossed by backcross and selfed to B5F4, With the specific gene Xa2 3 corresponding to the special germline P6 artificial inoculation identification, simultaneous selection of plant type and agronomic traits, until the stability of resistance and agronomic traits similar to the recurrent parent. The anti-spectrum of 20 representative strains of Philippine race, 10 Japanese races and 7 Chinese pathogens were compared between CBB2 3 (carrying Xa2 3) and IRBB2 1 (carrying Xa2 1) Resistant to all 20 bacterial strains; Xa21 against 19 bacterial strains, but highly susceptible to the Philippines, race # 10. The F2 segregation population of JG30 / CBB2 3 was constructed by using the near isogenic line CBB2 3. The SSR marker was used to screen Xa2 3 in rice chromosome 11. Three further AFL P markers were screened for closer linkage to Xa2 3. The distance between APKj2 3 and Xa2 3 is about 1.0 cM. It was also reported that the near-isogenic line CBB2 3 carrying Xa 2 3 has been effectively used in resistance breeding.