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本研究旨在探讨小金海棠多酚(Malus xiaojinensis polyphenols,MXP)的降脂减肥作用及其机制。采用饲喂高脂饲料建立高脂小鼠(Mus musculus)模型,小金海棠多酚灌胃给药(50,100 mg/kg),连续7周。检测血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)和肝组织中TG与TC水平,并计算肝脾指数。同时检测肝组织中白细胞介素-6(interleukin-6,IL-6)与肿瘤坏死因子(tumor necrosis factor-α,TNF-α)转录情况并观察肝组织形态。最后检测糖脂代谢途径相关蛋白胰岛素受体底物(insulin receptor substrate,IRS)和蛋白激酶B(Akt)的表达状况。结果显示,MXP可以显著抑制小鼠体重快速增长并降低血清和肝组织中TG、TC及LDL-C的水平,同时提高HDL-C的水平。此外,MXP能显著抑制包括IL-6及TNF-α在内的炎性细胞因子的生成及肝细胞损伤。更为重要的是MXP可以显著提高IRS-1和Akt的表达。因此,小金海棠多酚的降脂功能很可能与其能提高葡萄糖的利用率和抑制炎性细胞因子活性有关,具有开发成为降脂减肥功能食品及保健品的潜力。本研究为科学合理开发小金海棠(Malus xiaojinensis)提供理论基础和依据。
The aim of this study is to investigate the mechanism of lipid-lowering and weight-reducing in Malus xiaojinensis polyphenols (MXP). The model of high-fat mice (Mus musculus) was established by feeding high-fat diet. The small polygonatum was orally administered (50, 100 mg / kg) for 7 weeks. Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol HDL-C) and liver tissue TG and TC levels, and calculate the index of liver and spleen. At the same time, the transcription of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in liver tissue were detected and the morphology of liver was observed. Finally, the expression of insulin receptor substrate (IRS) and protein kinase B (Akt) in glucose and lipid metabolism pathway were detected. The results showed that MXP can significantly inhibit the rapid growth of mice weight and reduce serum and liver TG, TC and LDL-C levels, while increasing HDL-C levels. In addition, MXP significantly inhibited the production of inflammatory cytokines, including IL-6 and TNF-α, and hepatocellular injury. More importantly, MXP significantly increased the expression of IRS-1 and Akt. Therefore, the hypolipidemic effect of polygonum microphylla is likely to be related to its ability to increase glucose utilization and inhibit the activity of inflammatory cytokines, and has the potential to be developed into a lipid-lowering and weight-loss food and health supplement. This study provides the theoretical basis and basis for the scientific and rational development of Malus xiaojinensis.