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目的用流式细胞仪研究As2O3及NaAsO2诱导EBL7404细胞及HeLa细胞分化。方法采用0.25μmol/L的As2O3及NaAsO2同EBL7404细胞及HeLa细胞共同作用7 d后,用CD44作为宫颈及肝癌细胞的特异性抗原进行标记,应用流式细胞仪进行肿瘤细胞与分化细胞的分选。结果 0.25μmol/L的As2O3及NaAsO2同EBL7404细胞及HeLa细胞共同作用7 d后均有肿瘤细胞进行了不同程度的分化。以0.25μmol/L NaAsO2处理的HeLa细胞分化率最高,为1.8%,其次为0.25μmol/L As2O3处理的HeLa细胞,分化率为0.7%,0.25μmol/L As2O3处理的EBL7404细胞为0.6%,0.25μmol/LNaAsO2处理的EBL7404细胞分化率为0.3%。结论低剂量的As2O3及NaAsO2同EBL7404细胞及HeLa细胞共同作用一段时间后,均有不同细胞分化。
Objective To investigate the differentiation of EBL-7404 cells and HeLa cells induced by As2O3 and NaAsO2 by flow cytometry. Methods As2O3 and NaAsO2 were combined with EBL7404 cells and HeLa cells for 7 days and then labeled with CD44 as a specific antigen for cervical and hepatocellular carcinoma. Flow cytometry was used to sort tumor cells and differentiated cells . Results As2O3 and NaAsO2 (0.25μmol / L) had different degrees of differentiation with EBL7404 cells and HeLa cells for 7 days. HeLa cells treated with 0.25μmol / L NaAsO2 had the highest differentiation rate of 1.8%, followed by HeLa cells treated with 0.25μmol / L As 2 O 3 at a rate of 0.7%, 0.6% and 0.25% at 0.25μmol / L As 2 O 3 treatment The differentiation rate of EBL7404 cells treated with μmol / LNaAsO2 was 0.3%. Conclusion Low doses of As2O3 and NaAsO2 with EBL7404 cells and HeLa cells co-acting after a period of time, have different cell differentiation.