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目的:应用蒿芩清胆汤对流感病毒性肺炎湿热症模型进行治疗,并探索其中可能存在信号通路,为评价其作用打下实验基础。方法:将小鼠随机分为正常组、病毒组(单纯病毒感染)、模型组(高脂饮食+湿热环境+病毒感染)、阳性药物组(模型组+病毒唑)、蒿芩清胆汤组(模型组+蒿芩清胆汤)。造模成功后取小鼠肺组织,用免疫组织化学法检测肺组织切片NF-κB阳性光密度值;提取小鼠腹腔巨噬细胞用RT-PCR的方法检测其NF-κBmRNA的表达,测定各组小鼠肺指数并计算肺指数抑制率,再进行比较。结果与模型组相比,蒿芩清胆汤组的小鼠肺病变程度明显好转,肺组织NF-κB的阳性表达及腹腔巨噬细胞NF-κBmRNA的表达均明显降低,差异具有显著性(P<0.05)。结论:蒿芩清胆汤对流感病毒性肺炎湿热症疗效显著,而它是通过下调NF-κB的表达来干预肺泡内炎症反应,减少肺组织损伤从而达到治疗的目的。
OBJECTIVE: To treat the influenza virus pneumoconiosis model by using Artemisia Qingdan Decoction, and to explore the possible existence of signal pathways, which lays an experimental basis for the evaluation of its effects. Methods: The mice were randomly divided into normal group, virus group (simple viral infection), model group (high fat diet + hot and humid environment + virus infection), positive drug group (model group + ribavirin), Haoqinqingdantang group (Model group + Artemisia Galliformis soup). After the model was successfully established, the lung tissue of the mice was taken. The NF-κB positive optical density of the lung tissue sections was detected by immunohistochemical method; the peritoneal macrophages were extracted and the expression of NF-κB mRNA was detected by RT-PCR. The lung index of mice was calculated and the lung index inhibition rate was calculated and compared. Results Compared with the model group, the degree of lung lesions of the mice in the Artemisia Begoniae Decoction group was significantly improved, the positive expression of NF-κB in the lung tissue and the expression of NF-κB mRNA in the peritoneal macrophages were significantly reduced, and the difference was significant (P < 0.05). <0.05). Conclusion: Artemisia sinensis Qingdan decoction has a significant effect on influenza virus pneumoconiosis, and it is through the down-regulation of NF-κB expression to intervene in the alveolar inflammatory response, reduce lung tissue damage and achieve therapeutic goals.