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为了初步筛选出适宜诱导甜瓜红心脆、早皇后子叶和下胚轴愈伤组织和不定芽的培养基,以及筛选适宜其不定芽伸长和生根的培养基,采用组织培养的方法,以甜瓜红心脆和早皇后种子为材料、子叶和下胚轴为外植体,进行添加不同浓度6-苄氨基腺嘌呤(6-BA)、吲哚乙酸(IAA)激素组合的MS培养基诱导愈伤组织和不定芽、不定芽伸长、芽生根的研究。试验结果表明,MS+0.5 mg/L IAA、MS+1.0 mg/L IAA培养基不利于红心脆子叶愈伤组织的诱导,其余培养基均有利于其子叶和下胚轴愈伤组织的形成;MS和MS+0.3 mg/L IAA培养基不利于早皇后子叶愈伤组织的形成,MS培养基不利于其下胚轴愈伤组织的诱导,其余培养基均有利于其愈伤组织的形成;适宜诱导红心脆子叶不定芽的培养基是MS+0.5 mg/L 6-BA+1.0 mg/L IAA、MS+1.0 mg/L 6-BA+0.5 mg/L IAA,适宜诱导早皇后子叶不定芽培养基为MS+2.0 mg/L 6-BA+0.3 mg/L IAA;适宜诱导红心脆、早皇后下胚轴不定芽的培养基分别为MS+1.0 mg/L 6-BA+0.3 mg/L IAA、MS+0.3 mg/L 6-BA+0.5 mg/L IAA;MS+0.5 mg/L 6-BA、MS+1.0 mg/L IAA培养基分别适宜红心脆、早皇后不定芽伸长;2个品种适宜的生根培养基均是1/2MS+1.0 mg/L IBA。研究初步建立了甜瓜红心脆、早皇后的再生体系,为其遗传转化研究作了准备。
In order to preliminarily select the suitable medium for inducing the melon red heart crisp, the early cotyledon and hypocotyl callus and the adventitious bud, and selecting the medium suitable for the elongation and rooting of adventitious buds, the method of tissue culture, The seeds of brittle and early queen-seed, cotyledon and hypocotyls were used as explants. MS medium supplemented with 6-benzyladenine (6-BA) and indoleacetic acid (IAA) And adventitious buds, adventitious buds elongation, bud rooting research. The results showed that MS + 0.5 mg / L IAA and MS + 1.0 mg / L IAA were not conducive to the induction of Cotyledon centipede callus, while the rest of the medium was conducive to the formation of cotyledon and hypocotyl callus. MS and MS + 0.3 mg / L IAA medium was not conducive to the formation of early cotyledon callus, MS medium is not conducive to its hypocotyl callus induction, the rest of the medium are conducive to the formation of callus; The medium suitable for inducing adventitious buds of C. crispus was MS + 0.5 mg / L 6-BA + 1.0 mg / L IAA and MS + 1.0 mg / L 6-BA + 0.5 mg / L IAA Medium for MS + 2.0 mg / L 6-BA + 0.3 mg / L IAA; medium suitable for inducing red heart crisp, adventitious buds of early queen hypocotyls were MS + 1.0 mg / L 6-BA + 0.3 mg / L IAA, MS + 0.3 mg / L 6-BA + 0.5 mg / L IAA, MS + 0.5 mg / L 6-BA and MS + 1.0 mg / L IAA respectively. The appropriate rooting medium for each cultivar was 1 / 2MS + 1.0 mg / L IBA. The preliminary establishment of a melon red heart crisp, early queen regeneration system, prepared for its genetic transformation research.