目的探讨建立利用压力循环技术提取骨骼DNA的新方法。方法将11个不同部位骨骼样品分成3组,一组采用本实验室常规方法处理骨骼,一组采用压力循环仪处理骨骼,另外一组作为阴性对照,然后统一进行DNA的提取及定量。结果采用压力循环技术提取骨骼DNA时间比实验室常规方法缩短24小时以上,提取效率提高6%～49%。结论压力循环技术可以作为一种提取骨骼DNA提取的新方法之一。 Objective To explore a new method of extracting bone DNA using pressure cycling technique. Methods Bone samples from 11 different sites were divided into three groups. One group used the conventional method in our laboratory to treat the bones. One group used the pressure cycler to treat the bones and the other group served as the negative control group. Then DNA extraction and quantification were performed uniformly. Results The time of extraction of bone DNA by pressure cycle technology was shortened by more than 24 hours compared with the conventional method in the laboratory, and the extraction efficiency increased by 6% -49%. Conclusion Pressure cycling can be used as a new method to extract bone DNA.