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目的:建立HPLC法测定鹿胎软胶囊中4种嘌呤的含量。方法:色谱条件:流动相为0.02 mol/L KH2PO4缓冲液含有1 mmol/L庚烷磺酸钠(pH=3.8)∶甲醇=97∶3,检测波长254 nm,流速1.5 mL/min。4种嘌呤的线性关系:次黄嘌呤:Y1=83695X1+355(r1=0.9998),线性范围:0.040~0.667 mg/mL;黄嘌呤:Y2=50638X2+39(r2=0.9989),线性范围:0.008~0.119 mg/mL;鸟嘌呤:Y3=30269X3-9562(r3=0.9924),线性范围:0.018~0.279 mg/mL;腺嘌呤:Y4=38975 X4-8671(r4=0.9989),线性范围:0.027~0.399 mg/mL。4种嘌呤的平均加样回收率分别为98.1%、98.6%、98.0%和97.5%,RSD分别为1.0%、0.4%、0.8%和0.6%。结果:3批鹿胎软胶囊中次黄嘌呤、黄嘌呤、鸟嘌呤和腺嘌呤的含量为116.5~132.0μg/粒、21.2~23.0μg/粒、48.6~54.3μg/粒和68.9~75.2μg/粒。结论:该方法操作简便、准确、重复性好,为鹿胎软胶囊中嘌呤的分离和定量分析提供了依据。
Objective: To establish an HPLC method for the determination of 4 purines in deer fetal soft capsules. Methods: Chromatographic conditions: the mobile phase consisted of 0.02 mol / L KH2PO4 buffer containing 1 mmol / L sodium heptane sulfonate (pH = 3.8): methanol = 97: 3, detection wavelength 254 nm and flow rate 1.5 mL / min. Hypoxanthine: Y1 = 83695X1 + 355 (r1 = 0.9998), linear range: 0.040-0.667 mg / mL; xanthine: Y2 = 50638X2 + 39 (r2 = 0.9989) with a linear range of 0.008 Linear range: 0.018-0.29 mg / mL; adenine: Y4 = 38975 X4-8671 (r4 = 0.9989), linear range: 0.027 ~ 0.399 mg / mL. The average recoveries of four purines were 98.1%, 98.6%, 98.0% and 97.5%, respectively, with RSDs of 1.0%, 0.4%, 0.8% and 0.6%, respectively. Results: The contents of hypoxanthine, xanthine, guanine and adenine in three batches of deer fetal soft capsules were 116.5-132.0 μg / tablet, 21.2-23.0 μg / tablet, 48.6-54.3 μg / tablet and 68.9-75.2 μg / grain. Conclusion: The method is simple, accurate and reproducible. It provides the basis for the isolation and quantitative analysis of purine in deer fetal soft capsules.