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免疫治疗是肿瘤患者手术后预防肿瘤转移的较有效的手段之一,其中通过增强肿瘤的免疫原性,诱导机体产生特异性的抗肿瘤免疫来选择杀伤肿瘤细胞是免疫治疗的主要机理.本文通过黑色素瘤细胞经MHC I类基因转染及合用病毒感染后改变肿瘤细胞的免疫原性的方法制成瘤苗,来研究其抗肿瘤转移的免疫治疗效果.本实验将2×10~5B16F_(10.9)细胞接种于C57BL/6J(H-2~b)小鼠后,于不同时期分别用经基因转染或NDV感染的肿瘤细胞F_(10.9)-K~b、F_(10.9)-D~b、F10.9-NDV及联合基因转染后再感染NDV的瘤苗治疗荷瘤小鼠,结果发现:经F_(10.9)-K~b早期治疗(荷瘤11天)的荷瘤小鼠,其肿瘤转移率明显低于野生B16瘤苗及F_(10.9)D~b瘤苗治疗组,而感染NDV后制成的各种瘤苗均可有效地降低B16的肺转移、从防治晚期荷瘤小鼠的效果看,F_(10.9)-K~b可明显降低荷瘤18～25天时的小鼠的肺转移、而K~b-NDV-F_(10.9)瘤苗还可明显降低荷瘤32天的小鼠的肺转移,表明MHC H-2K基因疗法具有明显的抗肿瘤转移作用,同时也表明二者具有协同增强作用.作者进一步探讨了感染NDV的瘤苗产生作用的免疫机理,发现用F_(10.9)NDV瘤苗治疗手术治疗后的荷瘤小鼠,60%可存活至75～120天,其中30 %的小鼠在手术后120天解剖时,肺部无转移灶,平均肺重量为250±150mg,而对?
Immunotherapy is one of the most effective means to prevent tumor metastasis after surgery in cancer patients. Through enhancing the immunogenicity of the tumor and inducing the body to produce specific anti-tumor immunity, it is the main mechanism of immunotherapy to select tumor cells for killing tumors. Melanoma cells were transfected with MHC class I genes and infected with virus to change the immunogenicity of tumor cells. Tumor vaccines were made to study the immunotherapy effects of antitumor metastasis. This experiment will be 2×10~5B16F_(10.9). After the cells were inoculated into C57BL/6J (H-2~b) mice, the tumor cells infected with the gene or NDV were used at different times. F_(10.9)-K~b, F_(10.9)-D~b The tumor vaccines infected with NDV after transfection of F10.9-NDV and combined gene were used to treat tumor-bearing mice. The results showed that tumor-bearing mice treated with F_(10.9)-K~b early treatment (with tumor for 11 days), The tumor metastasis rate was significantly lower than that of wild B16 tumor vaccine and F_(10.9)D~b tumor vaccine treatment group, and various tumor vaccines made after infection with NDV can effectively reduce B16 lung metastasis and prevent and treat late tumors. The effect of mice, F_(10.9)-K~b can significantly reduce the lung metastasis in mice bearing 18 to 25 days of tumor, and K~b-NDV-F_(10.9) tumor vaccine It can significantly reduce the lung metastasis of tumor-bearing mice for 32 days, indicating that MHC H-2K gene therapy has a significant anti-tumor metastasis effect, and it also shows that the two have a synergistic effect. The author further explored the role of tumor vaccines infected with NDV The immune mechanism found that 60% of tumor-bearing mice treated with F_(10.9) NDV tumor vaccine survived to 75 to 120 days, of which 30% of mice were dissected 120 days after surgery. Metastases, with an average lung weight of 250±150 mg, and right?