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目的研究ＴＧＦα，ＥＧＦＲ反义寡聚核苷酸抑制大肠癌ＨＲ８３４８细胞株恶性增殖的作用．方法采用２３个碱基组成的ＴＧＦα反义寡聚核苷酸和２１个碱基组成的ＥＧＦＲ反义寡聚核苷酸以及２１个碱基组成的对照寡聚核苷酸作用于人大肠癌ＨＲ８３４８细胞，通过细胞生长抑制实验，３ＨＴｄＲ掺入，ｍＲＮＡ斑点杂交及细胞周期分析以确定其对大肠癌细胞的增殖抑制作用和作用环节．结果ＴＧＦα，ＥＧＦＲ反义寡聚核苷酸与对照寡聚核苷酸相比较，均能有效抑制ＨＲ８３４８细胞的增殖（Ｐ＜００１），ＤＮＡ合成（Ｐ＜００１）和ｍＲＮＡ（Ｐ＜００１）的表达，并能有效地延缓ＨＲ８３４８细胞由Ｇ０／Ｇ１期向Ｓ期的转化，延缓了细胞的分裂增殖
Objective To study the effect of TGFα and EGFR antisense oligonucleotides on malignant proliferation of colorectal cancer HR8348 cell line. Methods Human colon cancer HR8348 was treated with 23 base TGFα antisense oligonucleotides, 21 bases EGFR antisense oligonucleotides and 21 bases control oligonucleotides. Cells, through cell growth inhibition experiments, 3H TdR incorporation, mRNA dot blot hybridization and cell cycle analysis to determine its inhibition of proliferation of colorectal cancer cells and its role in the process. Results Compared with control oligonucleotides, TGFα and EGFR antisense oligonucleotides could effectively inhibit the proliferation of HR8348 cells (P<001), DNA synthesis (P<001) and mRNA (P). The expression of <001) can effectively delay the transformation of HR8348 cells from G0/G1 phase to S phase, delaying the proliferation of cells.