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建立了用反相高效液相色谱法测定槲皮素、芦丁、桑色素、木犀草素和染料木素等黄酮类化合物的方法.色谱条件是:ZORBAX ECLIPSE XPB—C8 5μm(4.6mm×150mm)色谱柱,V甲醇:V水(含0.2%磷酸)=50:50的溶液为流动相,流速为0.8mL/min。检测波长260nm.结果表明槲皮素、芦丁、桑色素、木犀草素和染料木素能较好的分离,分别在0.04~20,0.03~15,0.02~20,0.03~15,0.02~16μg/mL的浓度范围内与峰面积成良好线性关系,检测限分别为12