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目的观察转染miR-34a模拟物的鼻咽癌CNE-2细胞对紫杉醇敏感性的变化。方法培养鼻咽癌CNE-2细胞,将鼻咽癌CNE-2细胞分为观察组、对照组以及单药组,3组细胞均分别经0、12.5、25、50、100、200、400、800 ng/ml紫杉醇处理,此外,观察组转染miR-34a模拟物,对照组转染无关序列,单药组不转染任何序列。MTT法检测各组鼻咽癌CNE-2细胞的增殖能力,Annexin V-FITC/PI法检测各组鼻咽癌CNE-2细胞的凋亡率。结果 MTT结果显示,观察组内不同浓度紫杉醇处理鼻咽癌CNE-2细胞的OD值与对照组和单药组比较,均明显下降(P均<0.05),对照组与单药组比较(P均>0.05);Annexin V-FITC/PI法结果显示,观察组内不同浓度紫杉醇处理鼻咽癌CNE-2细胞的凋亡率与对照组和单药组比较均明显增加(P均<0.05),对照组与单药组比较差异均无统计学意义(P均>0.05)。结论转染miR-34a模拟物的鼻咽癌CNE-2细胞对紫杉醇的敏感性增强。
Objective To observe the changes of paclitaxel sensitivity of CNE-2 cells transfected with miR-34a mimics. Methods CNE-2 cells were cultured and divided into three groups: observation group, control group and single drug group. CNE-2 cells were respectively treated with 0,12.5,25,50,100,200,400, 800 ng / ml paclitaxel. In addition, miR-34a mimics were transfected in the observation group, unrelated sequences were transfected in the control group, and no sequence was transfected in the single drug group. The proliferation of CNE-2 cells was detected by MTT assay and the apoptosis rate of CNE-2 cells was detected by Annexin V-FITC / PI assay. Results The MTT results showed that OD value of nasopharyngeal carcinoma CNE-2 cells treated with different concentrations of paclitaxel in the observation group was significantly decreased compared with the control group and the single drug group (P <0.05) (All P> 0.05). The results of Annexin V-FITC / PI assay showed that the apoptosis rate of CNE-2 cells treated with different concentrations of paclitaxel in the observation group was significantly increased compared with the control group and the single drug group (all P < , There was no significant difference between the control group and the single drug group (all P> 0.05). Conclusion The sensitivity of paclitaxel to CNE-2 cells transfected with miR-34a mimics increased.