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鱼类能量学研究通常用耗氧率(MO_2)表征其某一生理状态的代谢强度.为考察不同流量和时间对鱼类标准代谢率(SMR)测定的影响,本研究在水温(25±0.5)℃条件下选取80尾鱼体健康、体质量相近的中华倒刺鲃Spinibarbus sinensis幼鱼作为实验鱼,研究在不同流量和测定时间条件下中华倒刺鲃幼鱼SMR数据的变化规律及稳定性.实验共设定5个流量梯度(1L/h,2L/h,3L/h,4L/h,5L/h),每一流量连续测量6个时间点,每隔1h测定1次SMR.结果显示:随着流量(v)逐渐加快,实验鱼呼吸室与空白呼吸室的溶氧差值(ΔMO_2)呈指数型下降,二者的拟合方程为ΔMO_2=0.872 2 v~(-0.999 8)(R~2=0.994,p<0.001).不同流量条件下,中华倒刺鲃幼鱼ΔMO_2的变异系数差异明显(p<0.001);其中,在流量5L/h条件下,ΔMO_2的变异系数最大.设定流量对中华倒刺鲃幼鱼SMR测定无显著影响(p>0.05).当流量越大时,在不同测定时间下SMR重复性的比例趋于变小.在设定的流量范围内中华倒刺鲃幼鱼SMR无显著差异,考虑样本量的大小、实验鱼体质量及低氧敏感性,建议鱼类SMR测定的流量设置不宜过快,并且SMR测定时间介于末次摄食后24~72h之间.
Fish energy studies usually use the oxygen consumption rate (MO 2) to characterize the metabolic intensity of a certain physiological state.In order to investigate the effects of different flux and time on the determination of standard metabolic rate (SMR) of fish, ) ℃ 80 juvenile Spinibarbus sinensis juvenile with similar body health and body weight were selected as experimental fish to study the variation regularity and stability of SMR data of juvenile Chinese barbed juvenile under different flow rates and measuring time A total of 5 flow gradients (1L / h, 2L / h, 3L / h, 4L / h and 5L / h) were set in the experiment. Six time points were measured continuously for each flow and SMR was measured every 1h. The results showed that the difference of dissolved oxygen value (ΔMO_2) between the respiration chamber and the blank respiration chamber of the experimental fish decreased exponentially with the increase of the flow rate (v), and the fitting equation was ΔMO 2 = 0.872 2 v -0.999 8 (R 2 = 0.994, p <0.001) .At the same flow rate, the variation coefficient of ΔMO_2 was significantly different (p <0.001), and the coefficient of variation of ΔMO_2 was the highest at the flow rate of 5L / h The flow rate had no significant effect on the SMR measurement of juvenile Chinese spinefish (p> 0.05) .When the flow rate was larger, the proportion of SMR repeatability tended to change under different measuring time SMR was no significant difference in the flow rate range, considering the sample size, experimental fish body quality and hypoxia sensitivity, it is suggested that the flowrate of fish SMR should not be set too fast, and the SMR Determination of time range between the last feeding after 24 ~ 72h.