目的研究不同鱼种来源的硫酸鱼精蛋白原料药的质量情况。方法对目前在我国上市的硫酸鱼精蛋白原料的鱼种来源采用HPLC进行鉴别和含量测定,对氨基酸组分进行考察,采用电感耦合等离子质谱法检测重金属含量,并考察了不同鱼种来源硫酸鱼精蛋白原料中和肝素的能力。结果目前我国上市的硫酸鱼精蛋白原料主要为鲑鱼和鲱鱼来源。结论本实验为建立硫酸鱼精蛋白的HPLC鉴别及含量方法,完善其质量标准提供了理论依据。 Objective To study the quality of protamine sulphate raw materials from different species of fish. Methods The sources of fish species of protamine sulphate currently listed in our country were identified by HPLC and the content of heavy metal was determined by inductively coupled plasma mass spectrometry (HPLC-MS). The contents of heavy metals Protamine material neutralizes the ability of heparin. Results At present, the main sources of protamine sulfate in our country are salmon and herring. Conclusion This experiment provides a theoretical basis for the establishment of HPLC method for the determination of protamine sulfate and its content and improvement of its quality standard.