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苦瓜遗传背景复杂,采用分子标记技术分析和评价苦瓜育种新组合的亲缘关系,对苦瓜优良品种的选育至关重要。本研究利用SSR标记和SRAP标记对22份苦瓜育种新组合材料进行了亲缘关系的分析,结果显示,筛选出的8对SSR和l0对SRAP特异引物的扩增结果均具有较高的多态性信息含量,平均多态性百分率为72.71%和83.80%。聚类分析结果发现,SSR和SRAP标记均能有效揭示苦瓜的遗传多样性,2种标记的聚类结果一致性高,在GS为0.71和0.64时,SSR和SRAP均可将22份材料分为4类。2种标记综合能够更准确地分析22份材料的亲缘关系。在GS为0.66时,22份材料被分为5类,在2种标记聚类分析中,亲缘关系较远的BL组合、YS31、JC21和MY023都各自聚类,HY、GL和ZY组合又聚为一类,这与GL组合和ZY组合是HY组合的父母本的事实相吻合。本研究结果表明,采用2种标记对苦瓜育种新组合进行综合分析,能更准确地反映苦瓜材料间的亲缘关系。
The genetic background of bitter gourd is complex. Using molecular marker technology to analyze and evaluate the genetic relationship of the new combination of bitter gourd breeding is very important for the breeding of excellent bitter gourd varieties. In this study, we analyzed the genetic relationship of 22 new combinations of bitter melon breeding using SSR markers and SRAP markers. The results showed that the amplified results of the 8 pairs of SSRs and 10 pairs of SRAP-specific primers all showed high polymorphism Information content, the average percentage of polymorphisms was 72.71% and 83.80%. Cluster analysis showed that both SSR and SRAP markers could effectively reveal the genetic diversity of bitter melon, and the clustering results of the two markers were highly consistent. SSR and SRAP could classify 22 materials 4 categories. The combination of two markers enables a more accurate analysis of the kinship of 22 materials. At GS 0.66, 22 materials were classified into 5 groups. In the 2 marker cluster analysis, the BL combinations with more distantly related species, YS31, JC21 and MY023 all clustered, and the combinations of HY, GL and ZY were clustered This is consistent with the fact that the GL combination and the ZY combination are parents of the HY combination. The results of this study showed that the comprehensive analysis of the new combination of bitter melon breeding using two kinds of markers can more accurately reflect the genetic relationship between bitter melon materials.