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【目的】探索过氧化物酶体增殖剂(Peroxisome proliferators,PPs)对稻瘟病菌生长发育及致病性的影响。【方法】在6种不同的PPs诱导下,观察比较稻瘟病菌过氧化物酶体数量及相关基因表达、生长速率、孢子萌发、附着胞形成与致病性的差异。【结果】在不同的PPs诱导下,稻瘟病菌过氧化物酶体数量均呈现明显的增加,同时过氧化物酶体形成相关基因PEX8、PEX11、PEX14的表达量升高;PPs影响病菌菌丝生长、分生孢子萌发及附着孢形成,并导致致病性的减弱。其中,2,4-D与阿司匹林的抑制效果最为显著。同时,2,4-D与ASA对稻瘟病菌过氧化物酶体形成突变体Δpex5和Δpex7的生长抑制效果与野生菌株相比明显增加。【结论】首次将PPs类化合物用于模式丝状病原真菌稻瘟病菌的研究。研究发现6种PPs均能够引起过氧化物酶体的增殖,并可抑制稻瘟病菌生长发育,降低致病性。
【Objective】 To explore the effects of peroxisome proliferators (PPs) on the growth and pathogenicity of Magnaporthe grisea. 【Method】 Under the induction of six different PPs, the differences in the number of peroxisomes and the related gene expression, growth rate, spore germination, adherent cell formation and pathogenicity of Magnaporthe grisea were compared. 【Result】 The results showed that the number of peroxisome proliferate obviously increased under the different PPs induction, and the expression of peroxisome-related gene PEX8, PEX11 and PEX14 was increased; PPs affected the mycelium of pathogen Growth, conidial germination and attachment sporulation, and lead to reduced pathogenicity. Among them, 2,4-D and aspirin inhibit the most significant effect. At the same time, 2,4-D and ASA significantly inhibited the growth of Pyricularia grisea peroxidase-forming mutants Δpex5 and Δpex7 compared with wild strains. 【Conclusion】 For the first time, PPs were used to study the pathogenic filamentous pathogen fungus Magnaporthe grisea. The study found that all 6 kinds of PPs can cause peroxisome proliferation, and can inhibit the growth and development of Magnaporthe grisea, reducing pathogenicity.