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目的:观察食物中蛋白质的氨基与糖的醛基之间非酶性糖化反应的终产物晚期糖基化终产物对健康SD大鼠肾脏的影响。方法:实验于2005-03/07在南方医科大学肾内科实验室和南方医科大学附属南方医院动物实验中心完成。①分组及干预:选用健康雄性SD大白鼠40只,使用数字表法随机将大鼠分为两组:高晚期糖基化终产物饲料组,喂饲高晚期糖基化终产物饲料;低晚期糖基化终产物饲料组,喂饲低晚期糖基化终产物饲料。②高、低晚期糖基化终产物大鼠饲料的制作:普通大鼠饲料由广东省实验动物中心提供。高晚期糖基化终产物大鼠饲料加工方法:新鲜烤制的面包,取面包皮在烤箱烤180℃,2h,90℃,22h后,与普通大鼠饲料1∶1混合压制成颗粒饲料;低晚期糖基化终产物大鼠饲料:新鲜烤制的面包,取面包芯室温自然风干后与普通大鼠饲料1∶1混合后压制成颗粒饲料。③标本收集:分两批分别于干预后第2,3个月处死,收集24h尿液、腹主动脉抽血、留取部分肾组织。④检测指标:考马斯亮蓝法检测24h尿蛋白含量;自动生化分析仪测定肾功能、血脂、血糖及白蛋白水平;肾组织经固定、脱水、石蜡包埋、切片后行苏木精-伊红和PAS染色,观察组织病理改变。结果:大鼠40只均进入结果分析。①高晚期糖基化终产物饲料组大鼠血清及肾组织匀浆羧甲基赖氨酸含量自高于后第2个月即开始增加,至第3个月显著高于低晚期糖基化终产物饲料组大鼠(血清:16.1,7.8μg/L;肾组织匀浆:31.3,14.2μg/g;P<0.01)②至干预后第3个月,高晚期糖基化终产物饲料组大鼠24h尿蛋白含量显著高于低晚期糖基化终产物饲料组(54.54,11.51mg/24h,P<0.01)。③干预后第3个月肾组织病理学观察显示,高晚期糖基化终产物饲料组大鼠肾小球体积增大,系膜基质增生,表现为肾小球PAS阳性染色面积占整个肾小球面积之比值增大,显著高于低晚期糖基化终产物饲料组(肾小球体积:1.59×106,1.02×106mm3,P<0.05;系膜基质:0.87,0.53,P<0.05)。结论:高晚期糖基化终产物食物加重健康SD大鼠血循环和肾组织晚期糖基化终产物的潴留程度,增加肾组织损害,在肾小球肥大和硬化中起着关键性作用。长期食用富含晚期糖基化终产物的食物可能会对肾脏造成一定的损害。
OBJECTIVE: To observe the effects of advanced glycation end products (AGEs), the final product of non-enzymatic glycation reaction between protein amino groups and carbohydrate aldehydes, on the kidneys of healthy SD rats. Methods: The experiment was performed at the Laboratory of Nephrology, Southern Medical University and the Southern Hospital of Southern Medical University from March to July in 2005. Grouping and Intervention: Forty healthy male SD rats were selected and randomly divided into two groups: high advanced glycation end products feed group and high advanced glycation end products feed; the low late stage Glycated end product feed group fed low advanced glycation end products feed. ② high, low advanced glycation end products rat feed production: General rat feed by the Guangdong Provincial Experimental Animal Center. Advanced glycation end products rat feed processing methods: freshly baked bread, take the bread in the oven roasted 180 ℃, 2h, 90 ℃, 22h, with the normal rat feed 1: 1 mixture pressed into pellets; Low advanced glycation end products rat feed: freshly baked bread, take the core of the bread core room temperature and then dried with ordinary rat feed 1: 1 mixed compressed into pellets. ③ Specimen collection: Two batches were executed at the 2nd and 3rd month after the intervention respectively. Urine collected 24 hours and abdominal aorta were drawn for blood collection, and some renal tissues were collected. ④Detecting index: 24h urinary protein content was detected by Coomassie brilliant blue method; renal function, blood lipids, blood glucose and albumin were measured by automatic biochemistry analyzer; Renal tissue was fixed, dehydrated and embedded in paraffin, sliced and hematoxylin-eosin And PAS staining, histopathological changes observed. Results: All 40 rats were involved in the result analysis. (1) The content of carboxymethyl lysine in serum and kidney homogenate of high-advanced glycation end products feed group began to increase from the second month after injection, and was significantly higher than the low-late glycosylation until the third month The rats in the final product diet group (serum: 16.1, 7.8 μg / L; kidney homogenate: 31.3, 14.2 μg / g; P <0.01) The 24h urinary protein content of rats was significantly higher than that of low-advanced glycation end products (54.54,11.51mg / 24h, P <0.01). ③The third month after intervention, the histopathological examination showed that glomerular volume increased and mesangial matrix proliferated in the advanced glycation end products-fed group, which showed that the area of glomerular PAS positive staining accounted for the entire kidney The ratio of globular area increased significantly, significantly higher than that of the low advanced glycation end products diet group (glomerular volume 1.59 × 106,1.02 × 106mm3, P <0.05; mesangial matrix: 0.87,0.53, P <0.05). CONCLUSION: High advanced glycation end products food aggravates the retention of advanced glycation end products in the blood circulation and renal tissues of healthy SD rats, increases renal tissue damage and plays a key role in glomerular hypertrophy and sclerosis. Long-term consumption of foods rich in advanced glycation end products may cause some damage to the kidneys.