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目的分析hedgehog(Hh)信号通路中Ptch1和Gli1蛋白在胆管癌组织中的表达及其意义。方法采用免疫组织化学方法检测62例胆管癌组织及其癌旁组织中Ptch1及Gli1蛋白的表达情况,并分析其在胆管癌组织中的表达与患者年龄、肿瘤大小、分化程度、肿瘤部位、淋巴结转移状况、TNM分期、手术方式及患者术后生存时间的相关性。结果 Ptch1和Gli1蛋白在胆管癌组织中的表达阳性率分别为74.2%(46/62)和88.7%(55/62),高于其在癌旁组织中的表达阳性率〔14.5%(9/62)和9.7%(6/62)〕,其差异有统计学意义(P<0.05)。胆管癌组织中Ptch1和Gli1蛋白的表达与患者年龄、肿瘤大小及肿瘤部位均无相关性(P>0.05),而与手术方式、分化程度、淋巴结转移状况、TNM分期及患者术后生存时间具有相关性(P<0.05)。结论 Ptch1和Gli1作为Hh信号通路蛋白在胆管癌组织中呈高表达状态,参与了胆管癌的发生及发展,可能是胆管癌检测与治疗的理想靶标。
Objective To analyze the expression of Ptch1 and Gli1 in hedgehog (Hh) signaling pathway in cholangiocarcinoma and its significance. Methods The expressions of Ptch1 and Gli1 protein in 62 cases of cholangiocarcinoma and its adjacent tissues were detected by immunohistochemistry. The expressions of Ptch1 and Gli1 in cholangiocarcinoma were analyzed with age, tumor size, differentiation, tumor location, lymph node Metastasis, TNM staging, surgical methods and the patient’s survival time after surgery. Results The positive rates of Ptch1 and Gli1 in cholangiocarcinoma were 74.2% (46/62) and 88.7% (55/62), respectively, which were higher than those in paracancerous tissues (14.5%, 9 / 62) and 9.7% (6/62)], the difference was statistically significant (P <0.05). The expression of Ptch1 and Gli1 protein in cholangiocarcinoma had no correlation with age, tumor size and tumor location (P> 0.05), but had correlation with the operation method, differentiation, lymph node metastasis, TNM staging and postoperative survival time Correlation (P <0.05). Conclusions Ptch1 and Gli1, as Hh signaling pathway proteins, are highly expressed in cholangiocarcinoma and participate in the occurrence and development of cholangiocarcinoma, which may be an ideal target for the detection and treatment of cholangiocarcinoma.