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目的 :探讨幽门螺杆菌 (Hp)毒力基因cagA、vacA抗体与胃十二指肠疾病之间的关系。方法 :采用免疫印迹法检测 440例胃十二指肠疾病患者血清中的cagA、vacA抗体。结果 :cagA、vacA抗体在 440例患者中的检出率分别为 73 %、37.0 %。在慢性胃炎 (CG)、十二指肠球部溃疡 (DU)、胃癌 (GC)患者中 ,cagA、vacA抗体的阳性率分别为 62 .9% ,76 .1 %、96 .9%与 33 .0 %、31 .0 %、62 .5 % ;经u检验显示 :慢性胃炎组与十二指肠球部溃疡组比较 ,无明显差异。胃癌组与慢性胃炎组、十二指肠球部溃疡组比较 ,有显著性差异。结论 :本文通过患者血清中Hp抗体 (cagA和vacA)的检测 ,推知其cagA和vacA抗体的表达状况 ,可为胃十二指肠疾病的诊断提供依据 ,但不能作为区分Hp感染所致胃十二指肠疾病的单一指标
Objective: To investigate the relationship between Helicobacter pylori (Hp) virulence genes cagA, vacA antibody and gastroduodenal diseases. Methods: Serum cagA and vacA antibodies in 440 patients with gastroduodenal diseases were detected by Western blotting. Results: The detection rates of cagA and vacA in 440 patients were 73% and 37.0% respectively. The positive rates of cagA and vacA antibodies in patients with chronic gastritis (CG), duodenal ulcer (DU) and gastric cancer (GC) were 62.9%, 76.1%, 96.9% and 33% respectively .0%, 31 .0%, 62.5%; by u test showed: chronic gastritis group and duodenal ulcer group, no significant difference. Gastric cancer group and chronic gastritis group, duodenal ulcer group, there are significant differences. Conclusion: The detection of cagA and vacA antibodies in serum of patients with Hp antibodies (cagA and vacA) can provide a basis for the diagnosis of gastroduodenal diseases, but can not be used as a basis for distinguishing between gastric A single indicator of the disease of the duodenum