It seems that electron beam is an effective and widely used way for sterilization. Escherichia coil is as one of the important biological indicators for electron beam irradiation sterilization of medical devices. An important question that we have to solve is to standardize the application of this bacterium as biological indicator in the practice of radiation sterilization for medical devices. In this regard and in order to further in- It seems that electron beam is an effective and widely used way for sterilization. Escherichia coil is as one of the important biological indicators for electron beam irradiation sterilization of medical devices. An important question that we have to solve is to standardize the application of this bacterium as biological indicator in the practice of radiation sterilization for medical devices. In this regard and in order to further in-