论文部分内容阅读
用循环伏安法制备了聚甲基红膜修饰玻碳电极(PMRE/GCE)。研究了L-色氨酸(L-Trp)在该修饰电极上的电化学行为,探讨了不同缓冲溶液、pH以及扫描速率等的影响。实验表明:在pH=5.5的HAc-NaAc支持电解质中,L-Trp在PMRE/GCE上的电化学氧化反应是一不可逆过程,于0.783V(vs.SCE)处产生一灵敏的氧化峰,峰电流大大增加。氧化峰电流与L-Trp的浓度在2.0×10-6~1.0×10-3mol/L范围内呈良好的线性关系,检出限为8.0×10-7mol/L,样品检测平均回收率为100.16%。
Polymethyl red film modified glassy carbon electrode (PMRE / GCE) was prepared by cyclic voltammetry. The electrochemical behavior of L-tryptophan (L-Trp) on the modified electrode was investigated, and the effects of different buffer solutions, pH and scanning rate were discussed. Experiments show that the electrochemical oxidation of L-Trp on PMRE / GCE is an irreversible process in HAc-NaAc supported electrolyte at pH = 5.5, which produces a sensitive oxidation peak at 0.783V (vs.SCE) Current greatly increased. The peak current of oxidation was linear with the concentration of L-Trp in the range of 2.0 × 10-6 ~ 1.0 × 10-3mol / L with the detection limit of 8.0 × 10-7mol / L. The average recovery of the sample was 100.16 %.