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A contributory role of oxidative stress and protection by antioxidant nutrients have been suspected in cataract forma-tion.Ganoderic acid A(GAA),an effective lanostane triterpene,is widely reported as an antioxidant.The aim of this study is to invest-igate the potential effects of GAA on cataract formation.After lens epithelial cells(LECs)were exposed to UVB radiation for differ-ent periods,cell viability,apoptosis-related protein levels,malondialdehyde(MDA)and superoxide dismutase(SOD)activities were monitored.We found that cell viability,the Bcl-2/Bax ratio and SOD activity were increased,while Cleaved caspase-3 levels and MDA activity were decreased compared with those in UVB-impaired LECs after GAA treated.Furthermore,GAA activated PI3K/AKT in UVB-impaired LECs and effectively delayed the occurrence of lens opacity in vitro.In conclusion,these findings demonstrated that GAA exhibited protective functions in SRA01/04 cells and rat lenses against UVB-evoked impairment through elev-ating cell viability and antioxidant activity,inhibiting cell apoptosis,activating the PI3K/AKT pathway and delaying lens opacity.