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为初步探究钙、磷在酸铝胁迫之后对豆科植物及其根瘤菌共生结瘤的修复效应,本试验采用水培培养法,以紫花苜蓿和耐酸苜蓿根瘤菌91522为材料,探讨了钙磷对酸铝胁迫后苜蓿根瘤菌生长、存活及苜蓿结瘤、初生根伸长的影响.结果显示,5mmol/L Ca2+能够显著提高酸铝胁迫下耐酸根瘤菌的生长和存活,4μmol/L P素在Ca2+的基础上进一步提高根瘤菌存活率.在钙、磷共同存在的条件下,苜蓿初生根的伸长量比酸铝胁迫对照提高了5～7倍;在铝毒害之后,通过补充Ca2+和P,苜蓿结瘤数、瘤鲜重、现瘤时间、农艺性状得到了显著的恢复;高浓度(10mmol/L)Ca2+对苜蓿根瘤菌生长有更好效果,但对苜蓿结瘤效果则不如5mmol/L Ca2+.初步认为,钙磷可以通过提高根瘤菌在酸性环境下的存活、苜蓿根系生长以及参与根瘤菌与豆科宿主识别过程来恢复酸铝毒害后的苜蓿-根瘤菌共生结瘤过程.
In order to investigate the repair effect of Ca and P on the symbiotic nodulation of leguminous plants and their rhizobia after acid-aluminum stress, the hydroponics culture method was used in this experiment. With alfalfa and Alcaligenes ruthenica 91522 as materials, The results showed that 5mmol / L Ca2 + could significantly increase the growth and survival of Rhizobia spp. Under acid-aluminum stress. The effect of 4μmol / LP on the growth and survival of Rhizobium meliloti, Ca2 + on the basis of further increase rhizobia survival rate in the presence of calcium and phosphorus, alfalfa primary root elongation than the acid-aluminum stress control increased 5 to 7 times; aluminum poisoning, by Ca2 + and P , Alfalfa nodulation number, tumor weight, tumor time and agronomic traits have been significantly recovered; high concentration (10mmol / L) Ca2 + has better effect on alfalfa rhizobia growth, but the effect of nodulation on alfalfa is not as good as 5mmol / L Ca2 +. It is preliminarily believed that calcium and phosphorus can restore the symbiotic nodulation process of alfalfa and rhizobia after acid-aluminum toxicity by increasing rhizobia survival in acidic environment, root growth of alfalfa and participating in identification of Rhizobium and legume host.