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BF-30是金环蛇Cathelicidin基因编码的一种直链多肽,由30个氨基酸构成。基于细菌生物被膜与细菌耐药性的关系,研究了BF-30对铜绿假单胞菌和金黄色葡萄球菌生物被膜的抑制作用及机理。当BF-30浓度为1/2×MIC时,可有效的抑制铜绿假单胞菌及金黄色葡萄球菌生物被膜的形成。以铜绿假单胞菌PAO1为研究对象,进一步考察亚抑菌浓度的BF-30抑制生物被膜形成的作用机理。实验结果表明,BF-30能够有效减少PAO1胞外鼠李糖的含量,从而降低细菌形成生物被膜的能力,但对PAO1表面的疏水性并无影响。此外,亚抑菌浓度的BF-30可剂量依赖性的降低细菌群体感应系统中绿脓菌素的释放量而不影响细菌对过氧化氢的敏感性,表明BF-30可通过群体感应系统调节生物被膜的形成。因此,BF-30有望用于治疗细菌生物被膜引起的各类慢性感染疾病。
BF-30 is a straight-chain polypeptide encoded by the Cathelicidin gene and consists of 30 amino acids. Based on the relationship between bacterial biofilm and bacterial resistance, the inhibitory effect and mechanism of BF-30 on biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were studied. When the concentration of BF-30 was 1/2 × MIC, it could effectively inhibit the formation of Pseudomonas aeruginosa and Staphylococcus aureus biofilm. Taking Pseudomonas aeruginosa PAO1 as the research object, the action mechanism of BF-30 inhibiting the formation of biofilm was further investigated. Experimental results show that BF-30 can effectively reduce the extracellular rhamnose content of PAO1, thereby reducing the ability of bacteria to form biofilm, but has no effect on the hydrophobicity of PAO1 surface. In addition, the concentration of bacteriostasis BF-30 dose-dependently reduced the release of pyocyanin in the bacterial population sensing system without affecting bacterial sensitivity to hydrogen peroxide, indicating that BF-30 can be modulated by the quorum-sensing system Biofilm formation. Therefore, BF-30 is expected to be used for the treatment of bacterial biofilms caused by a variety of chronic infectious diseases.